文献类型：期刊文献

英文题名：Photodynamic-therapy Activates Immune Response by disrupting Immunity Homeostasis of Tumor Cells, which Generates Vaccine for Cancer Therapy

作者：Zheng, Yuanhong[1,2];Yin, Guifang[1,2];Le, Vanminh[1,2];Zhang, Anle[1,2];Chen, Siyu[3];Liang, Xin[1,2];Liu, Jianwen[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Pharm, 268,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, 268,130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Dept Oncol, Sch Med, Xin Hua Hosp, Shanghai 200092, Peoples R China

年份：2016

卷号：12

期号：1

起止页码：120

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000369438800003)】；

基金：This work was supported by National Natural Science Foundation of China (No. 81502540), Fundamental Research Fund for the Central Universities of China (No. 222201514333), Shanghai Committee of Science and Technology (No. 13140902300), Nano Science and Technology Special Funding of Shanghai Committee of Science and Technology (No.11nm0503700), the Shanghai Committee of Science and Technology [grant 11DZ2260600] and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：DAMPs; Immunogenic apoptosis; Antitumor vaccination; Hypericin; CD47

摘要：Photodynamic therapy (PDT), a regulatory approved cancer treatment, is reported to be capable of causing immunogenic apoptosis. The current data reveal PDT can cause the dysregulation of "eat me" and "don't eat me" signal by generating reactive oxygen species (ROS) -mediated endoplasmic reticulum (ER) stress. This dysregulation probably contribute to the increased uptake of PDT-killed Lewis lung carcinoma (LLC) cells by homologous dendritic cells (DCs), accompanied by phenotypic maturation (CD80(high), CD86(high), and CD40(high)) and functional stimulation (NOhigh, IL-10(absent)) of dendritic cells as well as subsequent T-cell responses. Morevover, C57BL/6 mice vaccinated with dendritic cells (DCs) pulsed with PDT-treated LLCs (PDT-DCs) or PDT-treated LLCs alone (PDT-LLCs) exhibited potent immunity against LLC tumors. In the current study, the PDT-induced immune response was characterized as a process related with the dysregulation of "eat me" signal and "don't eat me" signal, revealing the possibility for developing PDT into an antitumor vaccination strategy for personalized cancer immunotherapy.