文献类型：期刊文献

中文题名：电子嗅在线反馈控制毕赤酵母糖化酶发酵过程中甲醇浓度新方法的应用

英文题名：Application of the Electronic Nose on the Online Feedback Control of Methanol Concentration during Glucoamylase Fermentation Optimization by Pichia pastoris

作者：施慧琳[1];孙靖淳[1];张荣凯[1];高大启[1];王泽建[1];郭美锦[1];周礼勤[2];庄英萍[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心上海生物制造产业技术研究院,上海200237;[2]广西科学院生物研究所,南宁530007

年份：2016

卷号：36

期号：3

起止页码：68

中文期刊名：中国生物工程杂志

外文期刊名：China Biotechnology

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"863"计划资助项目(2015AA021005)

语种：中文

中文关键词：电子嗅;甲醇;在线监测;毕赤酵母;糖化酶

外文关键词：Electronic nose Methanol; Online determination; Pichia pastoris; Glucoamylase

摘要：探索了电子嗅传感仪直接通过发酵尾气进行发酵液中甲醇浓度在线检测的方法,建立了毕赤酵母表达糖化酶过程中甲醇浓度的自动化反馈补料控制模型,可准确实现发酵过程中甲醇浓度的精确控制;研究表明,当利用电子嗅将培养液中甲醇浓度稳定控制在(890±35)ppm水平下,发酵诱导培养到128h时目的蛋白糖化酶酶活达到了8 153U/ml,与甲醇浓度控制在(350±26)ppm时的发酵水平相比提升了48.8%。该方法具有无需前处理、与发酵液非接触、快速和准确性的优点,为提升工程酵母在工业发酵培养过程工艺的优化控制具有重要的指导作用。
A novel method for direct determining the broth methanol concentration online from the fermentation exhausted gas with electronic nose sensor, established an auto-feedback model for methanol concentration control during glucoamylase fermentation by genetic Pichia pastoris were investigated,the model could be used for precise control in the fermentation process. Methanol concentration controlling strategies through electronic nose showed that when the broth methanol concentration in stability controlled at（ 890 ± 35）ppm,the highest glucoamylase activity reached 8 153 U / ml at 128 h of fermentation,which was 48. 8% higher than that with the methanol concentration controlled at（ 350 ± 26） ppm. Online methanol detection and feedback control models needn＇t pre-treatment and contact with the broth,has the rapid and accuracy advantages. It will be approved as an effective and important role on the online methanol concentration analysis and feedback control on industrial enzyme or aim protein fermentation with genetic Pichia pastoris.