文献类型：期刊文献

英文题名：Nitrate-reducing community in production water of three oil reservoirs and their responses to different carbon sources revealed by nitrate-reductase encoding gene (napA)

作者：Feng, Wen-Wen[1];Liu, Jin-Feng[1];Gu, Ji-Dong[2];Mu, Bo-Zhong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn & Inst Appl Chem, Shanghai 200237, Peoples R China;[2]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2011

卷号：65

期号：7

起止页码：1081

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000296216700022)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41073055) and the 863 Program (Grant No. 2009AA063503).

语种：英文

外文关键词：Nitrate-reducing; napA functional gene; Oil reservoirs; Community shift; Organic carbon

摘要：The nitrate-reducing microbial community in oil reservoirs was examined by PCR using primers to amplify a segment of napA gene encoding for a subunit of the nitrate reductase, and the effects of different organic carbon additions on the nitrate-reducing community were also evaluated. The orders Rhodocyclales and Burkholderiales within Betaproteobacteria and Pseudomonadales within Gammaproteobacteria were recovered in production water of all three oil reservoirs. Amendment of organic acids promoted Enterobacteriales within Gammaproteobacteria and orders of Rhodocyclales, Pseudomonadales, and Burkholderiales, while alkanes favored the Rhizobiaceae family within Alphaproteobacteria and orders of Rhodocyclales and Pseudomonadales. Results indicated that the functional gene napA can be used as a valuable biomarker in analyzing the diversity of nitrate reducers in oil reservoirs. Nitrate-reducing microbial community shifts following the available carbon sources. Information about napA gene in oil reservoirs environment is scarce. This is the first study that combines molecular and culture-dependent approaches to reveal the diversity of the nitrate-reducing microbial community in production water of oil reservoirs by using the napA gene. (C) 2011 Published by Elsevier Ltd.