文献类型：期刊文献

英文题名：A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography

作者：Cai, Jianfeng[1];Cheng, Lingping[1];Zhao, Jianchao[1];Fu, Qing[1];Jin, Yu[1];Ke, Yanxiong[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China

年份：2017

卷号：1524

起止页码：153

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20174104264471);WOS:【SCI-EXPANDED(收录号:WOS:000415772100016)】；

基金：This work was supported by the Project of National Natural Science Foundation of China (81403100), Fundamental Research Funds for the Central Universities (222201717016) and Open Funding Project of State Key Laboratory of Microbial Metabolism.

语种：英文

外文关键词：Hydrophilic interaction liquid chromatography; Polyacrylamide; Carbohydrates; Alcohols

摘要：A hydrophilic interaction liquid chromatography (HILIC) stationary phase was prepared by a two-step synthesis method, immobilizing polyacrylamide on silica sphere particles. The stationary phase (named PA, 5 mu m dia) was evaluated using a mixture of carbohydrates in HILIC mode and the column efficiency reached 121,000 N m(-1). The retention behavior of carbohydrates on PA stationary phase was investigated with three different organic solvents (acetonitrile, ethanol and methanol) employed as the weak eluent. The strongest hydrophilicity of PA stationary phase was observed in both acetonitrile and methanol as the weak eluent, when compared with another two amide stationary phases. Attributing to its high hydrophilicity, three oligosaccharides (xylooligosaccharide, fructooligosaccharide and chitooligosaccharides) presented good retention on PA stationary phase using alcohols/water as mobile phase. Finally, PA stationary phase was successfully applied for the purification of galactooligosaccharides and saponins of Paris polyphylla. It is feasible to use safer and cheaper alcohols to replace acetonitrile as the weak eluent for green analysis and purification of polar compounds on PA stationary phase. (C) 2017 Published by Elsevier B.V.