文献类型：期刊文献

中文题名：大豆乳清中β-淀粉酶的超滤提取技术研究

英文题名：Extraction of β-amylase from the soybean whey wastewater by ultrafiltration technology

作者：陈超琴[1];赵黎明[1];蒋丽华[1];夏泉鸣[1]

机构：[1]华东理工大学发酵工业分离提取技术研发中心,华东理工大学食品科学与工程系,上海200237

年份：2012

卷号：33

期号：23

起止页码：240

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：β-淀粉酶;大豆乳清废水;超滤

外文关键词：β-amylase; soybean whey wastewater; ultrafiltration

摘要：大豆乳清废水中含有丰富的β-淀粉酶。通过超滤技术对乳清废水中的β-淀粉酶进行浓缩回收,研究了膜截留分子量(MWCO)、操作温度、料液pH对超滤效果的影响。结果表明,选用MWCO3ku的膜,在跨膜压差2bar、温度(25±3)℃、pH7的操作条件下,膜通量由初始的44L/(m2·h)衰减至23L/(m2·h),平均通量达到35L/(m2·h);β-淀粉酶的酶活力可达到101U/mL,料液的浓缩倍数为10倍,酶活浓缩倍数约为9倍,总酶活的回收率为92%,浓缩液较高的β-淀粉酶酶活力为下一阶段的分离纯化奠定了基础,为大豆深加工废水综合利用提供了依据。
Ultrafiltration technology was used to concentrate the β-amylase from the soybean whey wastewater which displays high β-amylase activity. The optimization of the UF process which involved the selection of membrane molecular weight cut off （ MWCO）, operating temperature and the pH of the feed was studied. Experiment results showed that polyethersulfone membrane with molecular weight cut off（MWCO）3ku could be applied to enrich the β- amylase signally at trans- membrane pressure （TMP） ： 2bar; pHT.0,25 ± 3℃, the flux decreased from 44L/（m^2 .h） to 23L/（ m^2 · h）, got an average flux of 35L/（ m^2 · h） .The β- amylase in the retentate got an activity of 101U/mL with 92% yield, with a volumetric concentration ratio of ]0 times, and the concentration ratio of enzyme activity was about 9 times.β-amylase in the retentate with a relatively high activity, which provided a good foundation for the further purification of the β-amylase.Meanwhile,this technology provided a new thought to make good use of the soybean whey wastewater for the Chinese manufacturers.