文献类型：期刊文献

中文题名：细胞RNA影像解析技术的研究进展

英文题名：The advances in cellular RNA imaging technology

作者：陈显军[1];左方婷[1];谢鑫[1];方梦悦[1];杨弋[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2021

卷号：33

期号：3

起止页码：312

中文期刊名：生命科学

外文期刊名：Chinese Bulletin of Life Sciences

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家重点研发计划(2019YFA0904800,2017YFA050400);国家自然科学基金项目(91857202,21937004,31971349)。

语种：中文

中文关键词：RNA成像;荧光原位杂交技术;生物酶修饰;分子信标;荧光蛋白-RNA结合蛋白;荧光RNA

外文关键词：RNA imaging;fluorescence in situ hybridization;enzymatic modification;molecule beacon;fluorescent protein-RNA binding protein;fluorescent RNA

摘要：RNA具有独特的三维结构和种类繁多的生物学功能,广泛参与细胞的各类生命活动,与多种疾病的发生与发展密切相关。长期以来,科学家们一直在追求可以对细胞中RNA的丰度和分布进行解析的技术。该文将对多种细胞RNA影像解析技术的研究进展进行介绍,并将对最有希望成为活细胞RNA理想标记成像技术的荧光RNA进行重点介绍,包括它的种类以及在活细胞RNA成像中的应用,同时还将探讨细胞RNA影像解析技术未来的发展方向以及面临的挑战。
RNAs exhibit unique three-dimensional structures and a variety of biological functions,and have been widely involved in diverse life activities and closely related to the occurrence and development of many diseases.During these years,scientists have been persuing technologies that can analyze the abundance and distribution of RNA in cells.In this review,we describe the recent advances in cellular RNA imaging technology,and focus on fluorescent RNA,which is the most promising tool as an ideal technology for RNA imaging in live cells,including its types and applications in RNA imaging,and meanwhile we discuss the direction and challenge of future development of cellular RNA imaging technology.