文献类型：期刊文献

英文题名：Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone iia and cryptotanshinone from salvia miltiorrhiza bunge as a case study

作者：Zhang, Min[1]; Ignatova, Svetlana[2]; Hu, Ping[3]; Liang, Qionglin[4]; Wang, Yiming[4]; Luo, Guoan[1,4]; Wu Jun, Frank[5]; Sutherland, Ian[2]

机构：[1] Modern Engineering Center for Traditional Chinese Medicine, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; [2] Brunel Institute for Bioengineering, Brunel University, Kingston Lane, Uxbridge, Middlesex UB8 3PH, United Kingdom; [3] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; [4] Institute of Biomedicine, Tsinghua University, Beijing 100084, China; [5] XiangCam TCM Research Centre Ltd. Unit 16, Meditrina, Babraham Research Campus, Cambridge CB22 3AT, United Kingdom

年份：2011

卷号：1218

期号：36

起止页码：6031

外文期刊名：Journal of Chromatography A

收录：EI(收录号：20113314246474)

语种：英文

外文关键词：Efficiency - Separation - Dichloromethane - Organic solvents - Water injection - Liquid chromatography - Planning - Purification

摘要：A strategy for the development of a green process using counter-current chromatography technology is presented in this paper. The strategy began with solvent system selection, followed by linear scale-up from an analytical to a preparative process with optimized operating parameters. A two-stage separation using a multi-injection method was performed with a solvent system of hexane-dichloromethane-methanol-water (4:0.75:4:1) for the 1st stage and a hexane-ethanol-water (4:2:2) for the 2nd stage. A 191.8. mg of tanshinone IIA was purified, with a 97% purity and 34.4% recovery and a 276.7. mg of cryptotanshinone was separated, with a 95% purity and 31.8% recovery from 2.1. g of crude extract. Process parameters (throughput, efficiency, environmental risk factor and general process evaluation) and mass factors (mass intensity, separation mass efficiency and greenness) of a target were developed for monitoring of the counter-current chromatography process. ? 2011 Elsevier B.V.