文献类型：期刊文献

英文题名：Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

作者：Tian, Jingye[1];Shi, Donglei[2];Zhang, Yanhui[1];Li, Xiaokang[2];Li, Xinming[2];Teng, Hao[1];James, Tony D.[3];Li, Jian[2];Guo, Yuan[1]

机构：[1]Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol, Minist Educ, Xian 710127, Peoples R China;[2]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Pharm, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2021

卷号：12

期号：40

起止页码：13483

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20214411093080);WOS:【SCI-EXPANDED(收录号:WOS:000698773700001)】；

基金：We gratefully appreciate the financial support from the National Natural Science Foundation of China (21977082 and 22037002), the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China (2020JC-38) and the Technology Plan Project of Xi'an (GXYD18.1). T. D. J. wishes to thank the Royal Society for a Wolfson Research Merit Award. Y. G. thanks the University of Orleans in France for the invited professor position.

语种：英文

外文关键词：Chlorine compounds - Computer circuits - Probes

摘要：Diagnosing aging for preventative intervention generally relies on the tracking of aging biomarkers in the resting state. However, the static marker levels are insufficient to fully evaluate aging, particularly given that the stress response capacity (SRC) decay is currently viewed as a critical feature of aging. Therefore, we have developed a dual-channel fluorescent probe ROKS capable of the logic-based visualization of thiophenol (stressor) and HOCl (thiophenol-activated stress response product) in vivo, which provides a new strategy from the time dimension to precisely assess the SRC of individuals under stress using the dual-channel fluorescence ratio. Using ROKS we observed that the SRC of live cells decayed with senescence, and that a higher SRC was found for young vs. aged Caenorhabditis elegans. As such, our study offers a promising strategy for the fluorescence-guided diagnosis of aging and paves the way for accurate evaluation of the efficacy of anti-aging drugs.