文献类型：期刊文献

英文题名：Injectable Mussel-Inspired highly adhesive hydrogel with exosomes for endogenous cell recruitment and cartilage defect regeneration

作者：Zhang, Fang-Xue[1];Liu, Peng[2];Ding, Wang[1];Meng, Qing-Bing[1];Su, Di-Han[1];Zhang, Qi-Chen[1];Lian, Rui-Xian[3];Yu, Bao-Qing[5];Zhao, Ming-Dong[4];Dong, Jian[1];Li, Yu-Lin[3];Jiang, Li-Bo[1]

机构：[1]Fudan Univ, Zhongshan Hosp, Dept Orthopaed Surg, Shanghai 200032, Peoples R China;[2]Sichuan Acad Med Sci & Sichuan Prov Peoples Hosp, Eastern Hosp, Dept Orthoped Surg, Chengdu 610110, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[4]Fudan Univ, Jinshan Hosp, Dept Orthopaed, Shanghai 201508, Peoples R China;[5]Fudan Univ, Shanghai Pudong Hosp, Dept Orthoped, Pudong Med Ctr, 2800 Gongwei Rd, Shanghai, Peoples R China

年份：2021

卷号：278

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20214110997527);WOS:【SCI-EXPANDED(收录号:WOS:000710782400002)】；

语种：英文

外文关键词：Adhesive hydrogel; Exosomes; Cell recruitment; Cartilage defect

摘要：In the early stage of osteoarthritis (OA), cartilage degradation in the surface region leads to superficial cartilage defect. However, enhancing the regeneration of cartilage defect remains a great challenge for existing hydrogel technology because of the weak adhesion to wet tissue. In the present study, an injectable mussel-inspired highly adhesive hydrogel with exosomes was investigated for endogenous cell recruitment and cartilage defect regen-eration. The hydrogel with high bonding strength to the wet surface was prepared using a crosslinked network of alginate-dopamine, chondroitin sulfate, and regenerated silk fibroin (AD/CS/RSF). Compared with commercial enbucrilate tissue adhesive, the AD/CS/RSF hydrogel provided a comparative lap shear strength of 120 kPa, with a similar gelation time and a higher capacity for maintaining adhesive strength. The AD/CS/RSF/EXO hydrogel with encapsulated exosomes recruited BMSCs migration and inflation, promoted BMSCs proliferation and dif-ferentiation. Most importantly, the AD/CS/RSF/EXO hydrogel accelerated cartilage defect regeneration in situ, and extracellular matrix remodeling after injection in rat patellar grooves. The exosomes released by the hydrogels could recruit BMSCs into the hydrogel and neo-cartilage via the chemokine signaling pathway. Our findings reveal an injectable and adhesive hydrogel for superficial cartilage regeneration, which is a promising approach for minimally treating cartilage defect with arthroscopic assistance.