文献类型：期刊文献

中文题名：猪血血活素的制备和性能

英文题名：Preparation and Characterization of Hemo Active Element

作者：常雅宁[1];俞建瑛[1];张唯[1];宋聿文[1]

机构：[1]华东理工大学应用生物学系,上海200237

年份：2007

卷号：33

期号：3

起止页码：327

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：20072910703225);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：猪血;血活素;制备;性能

外文关键词：porcine blood ; hemo active element ; facture; character

摘要：以猪血为材料,运用热变性和乙醇除杂蛋白,再经过旋转蒸发、离子交换、活性炭脱色、浓缩得到血活素产品,并对产品进行了性能分析。结果表明:该产品已基本无大分子蛋白,干物质含量在25.6 mg/mL。微量金属含量为铜0.04 mg/mL、锌0.003 2 mg/mL、铁0.4μg/mL。运用该产品代替小牛血清培养细胞,细胞生长较明显,虽然与小牛血清培养效果存在差距,但可看出该产品在细胞培养方面具有潜在的开发前途。
Porcine blood was used as material. By adopting heat denaturalization and using ethanol, the unnecessary proteins were eliminated. Then hemo active element was abstracted through rolling vapori-zation, ion-exchange, decolorization with active carbon and concentration. Then the character of the product was analyzed. Result showed that there were almost no macromolecules left. The amount of the dry substance was 25.6 mg/mL, and the microelements were copper 0.04 mg/mL.zinc 0. 003 2 mg/mL and iron 0.4 μg/mL. Application of the product to cell culture as the substitute of calf serum, the growth of the cells was obvious. In spite of some differences with calf serum cultivation this product has potential prospect in cell cultivation.