文献类型：期刊文献

英文题名：A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy

作者：Wu, Yan[2];Su, Meng[2];Zhang, Shihao[3];Xiao, Lan[5,6];Xiao, Yin[5,6,7];Zhang, Mengya[1];Bei, Ying[2];Li, Meiyun[2];Zhang, Fan[2];Yuan, Qi[2];Wu, Shiyong[3];Liu, Jianyong[8];Li, Haitao[8];Yuan, Xiaohuan[2,12];Li, Meng[4,11];Li, Yulin[3,10];Gao, Jie[1,9]

机构：[1]Naval Med Univ, Shanghai Changhai Hosp, Changhai Clin Res Unit, Shanghai, Peoples R China;[2]Mudanjiang Med Univ, Coll Life Sci, Mudanjiang, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat Minist Educ, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai, Peoples R China;[4]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Dermatol, Shanghai, Peoples R China;[5]Queensland Univ Technol, Ctr Biomed Technol, Sch Mech, Med & Proc Engn, Brisbane, Australia;[6]Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Australia;[7]Griffith Univ, Sch Med & Dent, Mount Gravatt, Qld, Australia;[8]Huazhong Univ Sci & Technol, Wuhan Union Hosp, Tongji Med Coll, Dept Vasc Surg, Wuhan, Peoples R China;[9]Naval Med Univ, Shanghai Changhai Hosp, Changhai Clin Res Unit, Shanghai 200433, Peoples R China;[10]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Sch Mat Sci & Engn, State Key Lab Bioreactor Engn,Minist Educat, Shanghai 200237, Peoples R China;[11]Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Dermatol, Shanghai 200010, Peoples R China;[12]Mudanjiang Med Univ, Coll Life Sci, Mudanjiang 157011, Peoples R China

年份：2023

卷号：230

外文期刊名：MATERIALS & DESIGN

收录：;EI(收录号：20231814048793);WOS:【SCI-EXPANDED(收录号:WOS:001041745400001)】；

基金：This research was supported by the National Natural Science Foundation of China (81903233, 81874250), the Central Finance Supports Local Colleges, Universities Talent Development Funding from Heilongjiang Provincial Department of Finance (2020GSP09), the Natural Science Foundation of Heilongjiang Province (LH2020H076), and Mudanjiang City Guiding Scientific and Technological Plan Projects (HT2022JG125).

语种：英文

外文关键词：Amides - Biomechanics - Bone - Cell culture - Cell proliferation - Endothelial cells - Mammals - Reinforcement - Rigidity - Stem cells - Surface plasmon resonance

摘要：The application of gelatin hydrogels is hampered by their poor mechanical properties, which are unfavorable for debridement and diabetic wound healing. Herein, bovine serum albumin (BSA)-bridged gelatin hydrogels biopotentiated by bone marrow mesenchymal stem cell (BMSC)-derived nanovesicles (NV), named NV@BSA-GEL, were fabricated by reinforcement of gelatin hydrogels with genipin and BSA in a one-pot, low-cost green approach based on amide bonding-guided crosslinking. Genipin introduction endowed the crosslinking of gelatin hydrogels with weak mechanical properties that were reinforced by BSA bridging to realize suitable mechanical properties for wound dressing, including excellent flexibility and appropriate rigidity. The bioactivity of NV released by the hydrogels resulted in efficient ROS scavenging and favorable immunomodulatory activity as well as promoted cell proliferation and migration of fibroblasts and vascular endothelial cells. Proteomic analysis confirmed that NV harbors a variety of growth factors that can promote wound healing. In vivo, the NV@BSA-GEL hydrogel had superior wound healing effects compared to BSA-GEL, including faster wound healing, stimulation of angiogenesis, contributing to an anti-inflammatory extracellular matrix, and promoting skin maturation, with no systemic toxicity. Thus, the combined merits of NV@BSA-GEL suggest that it has high potential as a new hydrogel dressing for the safe and efficient treatment of diabetic wounds. & COPY; 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://