文献类型：期刊文献

英文题名：Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system

作者：Lv, Xiaocheng[1];Chen, Dandan[2,6];Yang, Liecheng[2,6];Zhu, Ning[1,5];Li, Jingling[2,6];Zhao, Jian[4];Hua, Zhibi[1,5];Wang, Fu-Jun[1,3,5];Zhang, Leshuai W.[2,3,6]

机构：[1]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd, Shanghai 201203, Peoples R China;[2]Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou 215123, Peoples R China;[3]Zhejiang Reachall Pharmaceut Co Ltd, Dongyang 322100, Zhejiang, Peoples R China;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]Shanghai R&D Ctr Standardizat Chinese Med, Shanghai 201210, Peoples R China;[6]Soochow Univ, Jiangsu Higher Educ Inst, Collaborat Innovat Ctr Radiat Med, Suzhou 215123, Peoples R China

年份：2016

卷号：86

起止页码：28

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20235015202894);WOS:【SCI-EXPANDED(收录号:WOS:000374196100004)】；

基金：The authors would like to thank the National Natural Science Foundation of China (#81373950 and #11304212), the Natural Science Foundation of Jiangsu Province of China (BK20130279), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) for support.

语种：英文

外文关键词：Polysaccharide; Natural products; Immunoregulation; Cell irregularity; Nitrogen oxide; Macrophage

摘要：In this study, polysaccharides were isolated from Astragalus membranaceus, Ganoderma lucidum and Radix ophiopogonis and named APSII, GLPII and OGPII for comparison of their immunoactivities. MTT assay indicated that these polysaccharides increased the metabolic activity of Raw264.7 macrophages and induced cell differentiation to dendritic like cells. High content screening and mathematical modeling were used to quantify the cell irregularity, a hallmark of cell differentiation by polysaccharides. The results showed that GLPII increased cell irregularity, but APSII and OGPII had slightly less effects. Imaging analysis also revealed that polysaccharides inhibited cell proliferation while inducing the cell differentiation. In addition, APSII and GLPII but not OGPII induced NO production and enhanced cell phagocytic ability. Interestingly, inducible nitric oxide synthase inhibitor blocked polysaccharide-enhanced phagocytosis, indicating NO production is crucial for macrophages to acquire phagocytic ability, which was further confirmed by correlation studies. APSII and GLPII significantly promoted the maturation of macrophages by the increase in the expression of MHCII, CD40, CD80 and CD86, while OGPII had less effects. In summary, we have suggested a practical and economical method to quantify macrophage differentiation (irregularity) induced by polysaccharides for quality assurance and have found the role of NO production on macrophage phagocytic ability. (C) 2016 Elsevier B.V. All rights reserved.