文献类型：期刊文献

英文题名：A novel click lysine zwitterionic stationary phase for hydrophilic interaction liquid chromatography

作者：Guo, Hongyue[1];Liu, Renhua[1];Yang, Jinjin[1];Yang, Bingcheng[1];Liang, Xinmiao[1];Chu, Changhu[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：1223

起止页码：47

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20120314699220);WOS:【SCI-EXPANDED(收录号:WOS:000300030700007)】；

基金：We acknowledge the financial support from the National Natural Science Foundation of China (No. 21172072, 21072055) and the Shanghai Natural Science Foundation (No. 11ZR1408500).

语种：英文

外文关键词：Click lysine; Analysis; HILIC; beta-Lactam antibiotics; Cephalosporins; Carbapenems

摘要：A novel type of zwitterionic HILIC stationary phase was prepared by covalently bonding the L-azido lysine on silica gel via click chemistry. The key intermediate azido lysine was synthesized by transformation the amino group in L-Boc-lysine to corresponding azido group and subsequent removal of the N-protected group (Boc). Finally, the azido lysine was covalently bonded to silica beads by click chemistry to get click lysine. Its structure was confirmed by FT-IR and elemental analysis. The new stationary phase showed good HILIC characteristics, when it was applied to separate polar and hydrophilic compounds, such as organic acids, cephalosporins and carbapenems. Compared with the commercial stationary phases, such as Atlantics HILIC and ZIC-HILIC, click lysine displayed better or similar chromatographic behaviors. (C) 2011 Elsevier B.V. All rights reserved.