文献类型：期刊文献

英文题名：Highly efficient conversion of polysialoganglioside to GM1 with Brevibacterium casei as a microbial biocatalyst

作者：Wang, Xuedong[1]; Yin, Zhe[1]; Peng, Yanfeng[1]; Shen, Yaling[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China

年份：2005

卷号：23

期号：1

起止页码：29

外文期刊名：Biocatalysis and Biotransformation

收录：EI(收录号：2005219110603)

语种：英文

外文关键词：Carbon - Catalysis - Chromatographic analysis - Purification - Separation - Soils

摘要：A novel can bacterium can be used to convert polysialoganglioside to GM1 efficiently, was isolated from soil with ganglioside as the sole source of carbon. It was identified tentatively as Brevibacterium casei. In a typical experiment, 0.8 g of highly purified GM1 (93%) was obtained from 1.5 g free GM1 crude ganglioside after chromatographic separation. The overall yield of this procedure was estimated to be more than 50% (w/w). ? 2005 Taylor & Francis Group Ltd.