文献类型：期刊文献

英文题名：Biodiesel production from waste cooking oil using onsite produced purified lipase from Pseudomonas aeruginosa FW_SH-1: Central composite design approach

作者：Ali, Chaudhry Haider[1,2,3];Qureshi, Abdul Sattar[4];Mbadinga, Serge Maurice[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Mu, Bo-Zhong[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai, Peoples R China;[3]Univ Engn & Technol, Dept Chem Engn, KSK Campus, Lahore 54890, Pakistan;[4]Univ Sindh, Inst Biotechnol & Genet Engn, Jamshoro, Sindh, Pakistan

年份：2017

卷号：109

起止页码：93

外文期刊名：RENEWABLE ENERGY

收录：;EI(收录号：20171103442222);WOS:【SCI-EXPANDED(收录号:WOS:000400212500009)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41273084) and the 863 Program (Grant No.2013AA064403).

语种：英文

外文关键词：Biodiesel; Methanol; Lipase; Waste cooking oil; Transesterification; Response surface methodology

摘要：Increasing energy demands, decreasing fossil fuel resources, instability of crude oil prices and pollution problems have compelled to switch over bio-based fuel for transportation, for example, biodiesel. In present study, waste cooking oil (WCO) was evaluated as feedstock for biodiesel production using free lipase in liquid. The response surface methodology (RSM) was used to optimize the interaction between four factors: the reaction temperature, methanol-oil molar ratio, dosage of lipase as biocatalyst and rotational speed. Using this method, the model predicted the optimal conditions reaching up to 86% FAME yield with temperature 44.2 degrees C, methanol-oil molar ratio (3.05:1), amount of lipase 0.782 g and rotation speed of 170 rpm with incubation period of 24 h. The reactions carried out under optimized conditions confirmed the validity of the model. The use of WCO in lipase catalyzed process can diminish the cost of biodiesel production and will allow lowering the direct use of edible oils to produce biodiesel instead. Thus, present study shows great practical potential to replace fossil fuel with renewable fuel (biodiesel). (C) 2017 Elsevier Ltd. All rights reserved.