文献类型：期刊文献

英文题名：Type II chaperonin gene as a complementary barcode for 16S rRNA gene in study of Archaea diversity of petroleum reservoirs

作者：Liu, Yi-Fan[1,2];Mbadinga, Serge Maurice[1,2,4];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2017

卷号：123

起止页码：113

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20172603811095);WOS:【SCI-EXPANDED(收录号:WOS:000411307100014)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 41530318, 41373070), Shanghai Fundamental Research Program (15JC1401400), and the Fundamental Research Funds for the Central Universities of China (No. 222201717017).

语种：英文

外文关键词：Archaea; 16S rRNA gene; Petroleum reservoir; Type II chaperonin; Biomarker

摘要：Archaea is an important component of the microbial community in subsurface petroleum reservoirs. Here, we compare Type II chaperonin (thermosome) and 16S rRNA gene as phylogenetic markers for revealing archaea in petroleum reservoirs. Evaluation of barcode gap of 16S rRNA targets and thermosome targets within 221 Archaea genomes available showed that thermosome targets have a much larger barcode gap than 16S rRNA, indicating that thermosome would provide improved species resolution over the 16S rRNA target. We then applied this combined approaches to two samples obtained from deep subsurface petroleum reservoirs. In general, members affiliated with the family Archaeoglobaceae were abundant in both samples regardless of the method used. While methanogenic archaea (Methanobacteriaceae, Methanosarcinaceae, Methanocalculaceae) were detected predominantly by 16S rRNA sequencing, thermosome sequences, however, showed the presence of archaeal members within the unclassified Thermofilaceae and unclassified Desulfurococcales, suggesting significant discrepancies between the two approaches to our understanding of the archaeal community taxa in deep subsurface petroleum reservoirs. Given these, Type II chaperonin (thermosome) can be a potential complementary biomarker in addition to 16S rRNA gene analysis of microbial community composition and diversity for more detailed information. (C) 2017 Published by Elsevier Ltd.