文献类型：期刊文献

英文题名：Metabolic Reprogramming and Proteome Reallocation Accompany Loss of Respiratory Oscillations in Yeast Accelerostat

作者：Wang, Gaoya[1,2];Chen, Yong[1];Zhuang, Yingping[1,2];Wang, Guan[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao New Energy Shandong Lab, Qingdao, Peoples R China

年份：2026

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20262220787252);WOS:【SCI-EXPANDED(收录号:WOS:001776346400001)】；

基金：This work was financially supported by the National Key R&D Program of China (2024YFA0920300), Natural Science Foundation of China (22578122), Natural Science Foundation of Shanghai (25ZR1402110), the Taishan Scholars Program of Shandong Province (tspn202408281), the Explorers Program of Shanghai (Basic Research Funding) (25TS1401100) and the Jiangsu Provincial Major Science and Technology Special Project (BG2025044).

语种：英文

外文关键词：Biochemistry - Glucose - Growth rate - Metabolism - Physiology - Proteomics

摘要：Respiratory oscillations are a hallmark of glucose-limited yeast chemostats, yet how growth rate shapes their emergence and collapse remains unclear. Here, we combined accelerostat cultivation with quantitative metabolomics and proteomics to characterize the transition from oscillatory to non-oscillatory metabolism in Saccharomyces cerevisiae under aerobic, glucose-limited conditions. Respiratory oscillations were maintained at low growth rates, attenuated at intermediate rates, and no longer observed at higher rates, coinciding with the onset of ethanol formation. Metabolomics analysis showed that oscillatory dynamics were most pronounced in tricarboxylic acid cycle intermediates and trehalose, whereas glycolysis and the pentose phosphate pathway exhibited weaker oscillations and instead adjusted pool sizes with growth rate. Quantitative proteomics further indicated that loss of oscillations was accompanied by non-uniform proteome reallocation, including increased representation of translation, glycolysis, energy metabolism, and amino acid biosynthesis, together with reduced relative allocation to buffering and proteostasis-related functions. Together, these results indicate a growth rate-associated physiological transition in glucose-limited yeast, in which the disappearance of oscillatory behavior during accelerostat cultivation is associated with a shift from respiratory to respiro-fermentative metabolism and coordinated reorganization of the proteome.