文献类型：期刊文献

英文题名：Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription

作者：Feng, Ying[1,2];Wang, Yao[3,4];Wang, Xiangnan[5];He, Xiaohui[5];Yang, Chen[6];Naseri, Ardalan[7];Pederson, Thoru[8];Zheng, Jing[6];Zhang, Shaojie[7];Xiao, Xiao[9];Xie, Wei[3,4];Ma, Hanhui[5]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai, Peoples R China;[2]ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China;[3]Tsinghua Univ, Sch Life Sci, Ctr Stem Cell Biol & Regenerat Med, MOE Key Lab Bioinformat, Beijing, Peoples R China;[4]Tsinghua Peking Ctr Life Sci, Beijing, Peoples R China;[5]ShanghaiTech Univ, Sch Life Sci & Technol, Beijing, Peoples R China;[6]East China Univ Sci & Technol, Sch Pharm, Shanghai, Peoples R China;[7]Univ Cent Florida, Dept Comp Sci, Orlando, FL 32816 USA;[8]Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA USA;[9]East China Univ Sci & Technol, Sch Biotechnol, Shanghai, Peoples R China

年份：2020

卷号：21

期号：1

外文期刊名：GENOME BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000600100800002)】；

基金：This work was funded by National Natural Science Foundation of China (No. 31970591 to H. Ma) and the Shanghai Pujiang program (19PJ1408000 to H. Ma). The initial phase of this work by H.M. in the laboratory of T.P. was funded in part through the U.S. National Institutes of Health 4D Nucleome Initiative, grant U01-DA-040588 to Paul Kaufman and T.P. This study was also funded by the National Natural Science Foundation of China (31988101, 31830047, 31725018 to W. X.), the THU-PKU Center for Life Sciences (W. X.), the Beijing Municipal Science & Technology Commission (grant Z181100001318006 to W. X.). W. X. is an HHMI international research scholar.

语种：英文

摘要：IntroductionDespite the long-observed correlation between H3K9me3, chromatin architecture, and transcriptional repression, how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear.ResultHere, we develop EpiGo (Epigenetic perturbation induced Genome organization)-KRAB to introduce H3K9me3 at hundreds of loci spanning megabases on human chromosome 19 and simultaneously track genome organization. EpiGo-KRAB is sufficient to induce genomic clustering and de novo heterochromatin-like domain formation, which requires SETDB1, a methyltransferase of H3K9me3. Unexpectedly, EpiGo-KRAB-induced heterochromatin-like domain does not result in widespread gene repression except a small set of genes with concurrent loss of H3K4me3 and H3K27ac. Ectopic H3K9me3 appears to spread in inactive regions but is largely restricted from transcriptional initiation sites in active regions. Finally, Hi-C analysis showed that EpiGo-KRAB reshapes existing compartments mainly at compartment boundaries.ConclusionsThese results reveal the role of H3K9me3 in genome organization could be partially separated from its function in gene repression.