文献类型：期刊文献

英文题名：Pyroptosis Mediates Neutrophil Extracellular Trap Formation during Bacterial Infection in Zebrafish

作者：Chen, Weijie[1];Zhao, Jingjing[1];Mu, Di[1];Wang, Zhuang[1];Liu, Qin[1,2];Zhang, Yuanxing[1,3];Yang, Dahai[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2021

卷号：206

期号：8

起止页码：1913

外文期刊名：JOURNAL OF IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000637391200022)】；

基金：This work was supported by the National Key Research and Development Program of China (2018YFC0311300), the Science and Technology Innovation Program by the Science and Technology Commission of Shanghai Municipality (20ZR1415500), and the Fundamental Research Funds for the Central Universities (2222012016009).

语种：英文

摘要：The formation of neutrophil extracellular trap (NET) is a critical host defense when neutrophils migrate to infection sites. Pyroptosis is a newly identified programmed cell death, which is tightly regulated by inflammasome activation. However, the mechanism of pyroptotic signaling participating in NET production remains to be elucidated. In this study, the zebrafish larvae otic vesicle microinjection model was used to infect larvae with hemolysin-overexpressing Edwardsiella piscicida (EthA(+)), and a rapid migration of neutrophils to infection sites was observed. Intriguingly, EthA(+) infection effectively induced significant neutrophil membrane rupture in vivo, which was dependent on caspase-B (caspy2) and gasdermin Eb (GSDMEb) but not caspase-A or gasdermin Ea. Specifically, the EthA(+) E. piscicida infection induced pyroptosis along with NETosis in vitro, and depletion of either caspy2 or GSDMEb impaired NET formation in vivo. Consequently, inhibition of the caspy2-GSDMEb axis-gated NETosis impaired bacterial clearance in vivo. Altogether, these data provide evidence that teleost fish innate immune cells, including neutrophils, express features of pyroptosis that are critical for NETosis in teleost innate immunity.