文献类型：期刊文献

中文题名：纳米通道单分子检测低噪音电流放大器系统的研究

英文题名：A Low Noise Amplifier System for Nanopore-based Single Molecule Analysis

作者：颜秉勇[1];顾震[1];高瑞[1];曹婵[1];应佚伦[1];马巍[1];龙亿涛[1]

机构：[1]华东理工大学自动化系结构可控先进功能材料及其制备重点实验室,上海200237

年份：2015

卷号：43

期号：7

起止页码：971

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000357987600003)】；北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：This work was supported by by the National Natural Science Foundation of China (Nos. 21125522, 21327807, 51407078) and the Fundamental Research Funds for the Central Universities of China (Nos. 222201313004, WH1514049).

语种：中文

中文关键词：纳米通道;单分子检测;电流放大器;降噪

外文关键词：Nanopore;Single molecule detection;Current amplifier;Denoising

摘要：研制了一种新型微电流放大器系统,用于检测α-Hemolysin生物蛋白纳米通道在单分子检测实验中所产生的微弱电流信号(<100 p A)。在1 mol/L KCl、10 mmol/L Tris-HCl,1 mmol/L EDTA的缓冲液(p H 8.0)中测定了DNA-PEG-DNA交联物与纳米通道的穿越和碰撞信号。实验中使用3 k Hz贝塞尔滤波器和100 k Hz模数转换器来对电流进行采样。结果表明,此放大器系统能够有效降低电流记录过程中的噪音,有利于分辨待测物分子与纳米通道作用所产生的较小阻断的电流信号(<10 p A)。
A novel amplifier system was proposed for low-noise recording of pico-ampere current in nanopore experiment （〈100 pA）. As an example, the amplifier system was applied in α-hemolysin based nanopore detection of DNA-PEG-DNA conjugate to record the signals of translocation and bumping events in buffer solution （1 mol/L KCl, 10 mmol/L Tris--HCl, 1 mmol/L EDTA and pH=8. 0）. The amplified current signal was filtered by a 3 kHz Bessel filter and sampled by a 100 kHz analog-digital convertor. As a result, the presented amplifier system could lower the noise in recording the current. The current blockages （〈10 pA） of single molecules with low amplitude were recovered due to the high signal-to-noise ratio.