文献类型：期刊文献

英文题名：Preparation of open porous polycaprolactone microspheres and their applications as effective cell carriers in hydrogel system

作者：Zhang, Qingchun[1];Tan, Ke[2];Ye, Zhaoyang[2];Zhang, Yan[1];Tan, Wensong[2];Lang, Meidong[1]

机构：[1]Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Jiaozuo City, Peoples R China;[2]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：32

期号：8

起止页码：2589

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20123715437590);WOS:【SCI-EXPANDED(收录号:WOS:000310396900066)】；

基金：Financial support is from the Fundamental Research Funds for the Central Universities (WD0913008, WD1014036), the National Natural Science Foundation of China (20804015), "Shu Guang" Project of Shanghai Municipal Education Commission, Specialized Research Fund for the Doctoral Program of Higher Education (200802511021) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0825).

语种：英文

外文关键词：Polycaprolactone; Porous microspheres; Paraffin; Mesenchymal stem cells; Microsphere/hydrogel composite

摘要：Common hydrogel, composed of synthetic polymers or natural polysaccharides could not support the adhesion of anchorage-dependent cells due to the lack of cell affinitive interface and high cell constraint. The use of porous polyester microspheres as cell-carriers and introduction of cell-loaded microspheres into the hydrogel system might overcome the problem. However, the preparation of the open porous microsphere especially using polycaprolactone (PCL) has been rarely reported. Here, the open porous PCL microspheres were fabricated via the combined emulsion/solvent evaporation and particle leaching method. The microspheres exhibited porous surface and inter-connective pore structure. Additionally, the pore structure could be easily controlled by adjusting the processing parameters. The surface pore size could be altered from 20 mu m to 80 mu m and the internal porosities were varied from 30% to 70%. The obtained microspheres were evaluated to delivery mesenchymal stem cells (MSCs) and showed the improved cell adhesion and growth when compared with the non-porous microspheres. Then, the MSCs loaded microspheres were introduced into agarose hydrogel. MSCs remained alive and sustained proliferation in microsphere/agarose composite in 5-day incubation while a decrement of MSCs viabilities was found in agarose hydrogel without microspheres. The results indicated that the microsphere/hydrogel composite had a great potential in cell therapy and injectable system for tissue regeneration. (C) 2012 Elsevier B.V. All rights reserved.