文献类型：期刊文献

英文题名：Imaging the Redox States of Live Cells with the Time-Resolved Fluorescence of Genetically Encoded Biosensors

作者：Li, Lei[1,4];Zhang, Changcheng[2,3];Wang, Peng[1];Wang, Aoxue[2,3];Zhou, Jiasheng[1];Chen, Guoqing[4];Xu, Jianhua[1];Yang, Yi[2,5];Zhao, Yuzheng[2,3];Zhang, Sanjun[1,6,7];Tian, Yang[8]

机构：[1]East China Normal Univ, State Key Lab Precis Spect, 3663 North Zhongshan Rd, Shanghai 200062, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]Jiangnan Univ, Sch Sci, Wuxi 214122, Peoples R China;[5]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai 200031, Peoples R China;[6]Shanxi Univ, Collaborat Innovat Ctr Extreme Opt, Taiyuan 030006, Shanxi, Peoples R China;[7]NYU Shanghai, NYU ECNU Inst Phys, 3663 North Zhongshan Rd, Shanghai 200062, Peoples R China;[8]East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Dongchuan Rd 500, Shanghai 200241, Peoples R China

年份：2019

卷号：91

期号：6

起止页码：3869

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20191206672102);WOS:【SCI-EXPANDED(收录号:WOS:000462098300017)】；

基金：This work was supported by the National Science Foundation of China (11774096, 11674101, 6141001145, 31722033, 91649123, 31671484, and 21827814), the National Key Research and Development Program of China (2017YFA050400 and 2017YFC0906900), the 111 project (B12024), the JoRISS project, the National First-Class Discipline Program of Food Science and Technology (JUFSTR20180302), and the Fundamental Research Funds for the Central Universities (2050205).

语种：英文

外文关键词：Cells - Fluorescence - Cytology - Bioimaging

摘要：Redox environments in cells influence many important physiological and pathological processes. In this study, the time-resolved fluorescence of a recently reported thiol redox-sensitive sensor based on vertebrate fluorescent protein UnaG, roUnaG, was studied, along with the application of the time-resolved fluorescence of roUnaG to image the redox states of the mitochondria, cytoplasm, and nucleus in live cells. Time-resolved fluorescence images of roUnaG clearly demonstrated that potent anticancer compound KP372-1 induced extreme oxidative stress. A more stressful redox state observed in activated macrophages further demonstrated the validity of roUnaG with time-resolved fluorescence. For comparison, time-resolved fluorescence images of four other frequently used redox biosensors (roGFP1, HyPer, HyPerRed, and rxRFP) were also captured. The time-resolved fluorescence allows an intrinsically ratiometric measurement for biosensors with one excitation wavelength and provides new opportunities for bioimaging.