文献类型：期刊文献

英文题名：Molecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfields

作者：Li, Hui[2];Wang, Xiao-Li[3,4];Mu, Bo-Zhong[3,4];Gu, Ji-Dong[5];Liu, Yong-Di[1];Lin, Kuang-Fei[2];Lu, Shu-Guang[2];Lu, Qiang[2];Li, Bing-Zhi[2];Li, Yang-Yang[6];Du, Xiao-Ming[7]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Inst Appl Chem, Sch Bioengn, Shanghai 200237, Peoples R China;[5]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China;[6]Shanghai Res Inst Chem Ind, Shanghai 200237, Peoples R China;[7]Chinese Res Inst Environm Sci, Dept Soil Pollut Control, Beijing 100012, Peoples R China

年份：2013

卷号：76

起止页码：49

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20130215880417);WOS:【SCI-EXPANDED(收录号:WOS:000312759300010)】；

基金：This study was financially supported by the National Natural Science Foundation of China (40871223, 41003031, 41073055), the National Environmental Protection Public Welfare Science and Technology Research Program of China (200909089, 2010467032, 201109013), the Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), the Fundamental Research Funds for the Central Universities (WB1014004), the Major Science and Technology Program for Water Pollution Control and Treatment (2009ZX07101-009-03), the National Science Foundation for Postdoctoral Scientists of China (2012M510811), and the Postdoctoral Science Foundation of Shanghai (11R21412500). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：alkB gene; Alkane-degrading bacteria; Denaturing gradient gel electrophoresis (DGGE); Oil contamination; Bioremediation

摘要：Alkane-degrading bacteria are crucial in the bioremediation of petroleum contamination from soil and groundwater. The alkane monooxygenase (alkB) gene encoding the Alk enzyme involved in aerobic degradation is a potentially functional gene biomarker for the detection of alkane-degrading bacteria. This study describes a denaturing gradient gel electrophoresis (DGGE) assay to facilitate the rapid and sensitive detection of alkB genes in wastewater from oilfield. The results showed that the presence of a considerable genetic diversity of alkB genes in the wastewater as evidenced by a total of 13 unique DNA bands detected. These alkB genes belong to nine genera, including Acinetobacter, Alcanivorax, Acidisphaera, Burkholderia, Geobacillus, Marinobacter, Mycobacterium, Pseudomonas, Rhodococcus and Xanthobacter. The abundance of alkane-degrading bacteria and total bacteria was calculated to range from 1.46 x 10(3) to 9.89 x 10(4) cell ml(-1) and from 1.18 x 10(4) to 6.29 x 10(5) cells ml(-1), respectively. The distribution of alkane-degrading bacteria was positively correlated with the environmental temperature and the specific types of n-alkanes in the wastewaters. Our results suggest that alkB-based DGGE methods could describe the diverse alkane-degrading bacteria in oil-degrading community, which may. improve the understanding of the treatment for oil-polluted wastewater. (c) 2012 Published by Elsevier Ltd.