文献类型：期刊文献

英文题名：Mixed bacteria passivation for the remediation of arsenic, lead, and cadmium: Medium optimization and mechanisms

作者：Peng, Cheng[1];Zhao, Xuan[1];Ji, Xiaowen[1];Wu, Jinhong[3];Liang, Weiyu[1];Song, Huihui[1];Zhang, Wei[1];Wang, Xuedong[2]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asses, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Yaxin Urban Construction Co Ltd, Shanghai 200436, Peoples R China

年份：2023

卷号：170

起止页码：720

外文期刊名：PROCESS SAFETY AND ENVIRONMENTAL PROTECTION

收录：;EI(收录号：20225213306474);WOS:【SCI-EXPANDED(收录号:WOS:000910453600001)】；

基金：Acknowledgements This research was supported by National Key Research and Devel-opment Program of China (2019YFC1805203) and National Natural Science Foundation of China (21737005, 41877124, 42177395) .

语种：英文

外文关键词：Mixed bacteria; Bacillus; Optimization; Heavy metal; Bioremediation

摘要：Lead(Pb), cadmium(Cd), and arsenic(As) are highly bioaccumulative and hazardous to human health. In this study, the effectiveness and mechanism of mixed bacteria applied to Pb, Cd, and As complex contaminated soil were investigated. According to the one factor per time (OFT) method, the optimal medium consisted of 10 g/L corn pulp dry powder, 10 g/L glucose, 3.8 g/L dipotassium hydrogen phosphate, 0.5 g/L sulfate, 1 g/L trisodium citrate at pH 7.2. The gradient test showed that the key fermentation process conditions were the optimal inoculum level of 1% and incubation temperature of 37 celcius. Then the mixed bacteria agent was used to treat the Pb(II), Cd(II), and As(III) contaminated soil. The adsorption amounts of Pb(II), Cd(II), and As(III) by the mixed bacteria were 10.39 mu g/g, 1.65 mu g/g, and 67.03 mu g/g, respectively. The X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and X-ray photoelectron spectroscopy (XPS) analyses showed that the remediation mechanism of three metals by mixed bacteria involved in extra cellular polymer adsorption, biomineralization, and oxidation. This study would provide a potential remediation method for the multi-heavy metal polluted soil.