文献类型：期刊文献

英文题名：A highly selective and sensitive near-infrared fluorescence probe for arylamine N-acetyltransferase 2 in vitro and in vivo

作者：Wang, Xin[1];Cui, Lei[1];Zhou, Nannan[1];Zhu, Weiping[1];Wang, Rui[1];Qian, Xuhong[1];Xu, Yufang[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：4

期号：7

起止页码：2936

外文期刊名：CHEMICAL SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000319940400030)】；

基金：This work was financially supported by the National Basic Research Program of China (973 Program, 2013CB733700, 2010CB126100), the National Natural Science Foundation of China (Grant 21236002), the National High Technology Research and Development Program of China (863 Program 2011AA10A207), and the Fundamental Research Funds for the Central Universities.

语种：英文

摘要：We developed a near-infrared fluorescence probe for the highly selective and sensitive detection of NAT2 activity. An arylamine was designed as the substrate part for NATs, as well as a PET quencher for cyanine dye. CYP1 with a 4-position arylamine displayed efficient PET quenching, and exhibited high selectivity and sensitivity to NAT2 with no response to NAT1. For practical application, we demonstrated CYP1 could exhibit excellent performance for endogenous NAT2 fluorescence imaging in living mice. We further verified the NAT2 distribution in various tissues by detecting the response to the corresponding tissue homogenates. The results were consistent with the fluorescence imaging in vivo and previous research. Above all, the probe CYP1 could selectively and sensitively monitor the NAT2 activity in enzymatic system, living mice and tissue homogenate samples. Therefore, the results indicated that CYP1 has potential use for the detection of NAT2 activity in the clinic and in research.