文献类型：期刊文献

英文题名：Mixotrophic cultivation of Botryococcus braunii

作者：Zhang, Huifang[1];Wang, Weiliang[1];Li, Yuanguang[1];Yang, Wenjie[1];Shen, Guomin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：35

期号：5

起止页码：1710

外文期刊名：BIOMASS & BIOENERGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000290238200012)】；

基金：This research was funded by National Basic Research Program of China (973 Program: 2011CB200904), National High Technical Development Project (863 Project: 2007AA02Z209, 2007AA09Z419), the National Special Fund for State Key Laboratory of Bioreactor Engineering (Grant No. 2060204) and the Open Research Fund of Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource (2010F30003).

语种：英文

外文关键词：Botryococcus braunii; Mixotrophic cultivation; Glucose; Photobioreactor; Hydrocarbon

摘要：Cultivation of Botryococcus braunii has been mainly reported in photoautotrophic mode which has some disadvantages such as low cell growth rate and low cell density. For high density and high productivity, this study attempts to cultivate B. braunii in mixotrophic mode. Effects of different organic carbon sources (including maltose, glucose, saccharose, lactose, glycerol and starch), glucose and KNO(3) concentrations as well as inoculation amount on the growth of B. braunii were investigated in 250 mL shake flasks, and a fed-batch mixotrophic cultivation technique was developed in a 10 L enclosed automatic air-lift photobioreactor. B. braunii grew faster in mixotrophic cultivation with all the six organic carbon sources than that in photoautotrophic mode, and glucose was the optimum. The optimal concentrations of glucose and KNO(3) were 2.5 g L(-1) and 0.4 g L(-1) respectively. Within the inoculums of 46-200 g m(-3), the lag phase of cell growth was very short, and cells grew fast into exponential phase after inoculation, the average cell growth rate of B. braunii increased with the increment of inoculation amount. With a 10 L air-lift photobioreactor, B. braunii was cultured by feeding glucose under mixotrophic condition for 19 days, and the cell density and hydrocarbon content in dry cell reached 4.55 g L(-1) and 29.7%, respectively. (C) 2011 Elsevier Ltd. All rights reserved.