文献类型：期刊文献

中文题名：竹荪多糖提取工艺及抗氧化活性

英文题名：Extraction process of polysaccharide from Dictyophora indusiata and antioxidant activity

作者：卜原玲[1];姚泽远[1];韩伟[1];刘艳芳[2];李德顺[2]

机构：[1]华东理工大学药学院、制药工程与过程化学教育部工程研究中心、上海市新药设计重点实验室,上海200237;[2]上海市农业科学院、食用菌研究所、国家食用菌工程技术研究中心,上海201403

年份：2023

卷号：45

期号：2

起止页码：228

中文期刊名：南京工业大学学报（自然科学版）

外文期刊名：Journal of Nanjing Tech University(Natural Science Edition)

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：上海市科技兴农重点攻关项目[沪农科创字(2018)第11号]。

语种：中文

中文关键词：竹荪多糖;缓冻协同微波法;响应面;提取工艺;抗氧化活性;高效液相色谱联用多角度激光散射;红外光谱

外文关键词：polysaccharides of Dictyophora indusiata;slow freezing-microwave assisted extraction;response surface;extraction process;antioxidant activity;high-performance liquid chromatography multi-angle laser scattering(HPSEC-MALLS-RI);infrared spectroscopy

摘要：采用缓冻协同微波法对竹荪中的多糖成分进行提取并进行工艺研究,依次考察缓冻时间、微波功率、液料比、提取时间对多糖得率的影响,在单因素试验基础上,运用响应面设计优化得到最佳提取工艺。利用1,1二苯基2三硝基苯肼(DPPH)自由基清除法、2,2'联氮双(3乙基苯并噻唑啉6磺酸)二铵盐(ABTS)自由基清除法、羟自由基清除法、总还原能力4种试验,对比缓冻协同微波法提取得到的竹荪多糖(PW 1)和单一微波法获得的竹荪多糖(PW 2)的抗氧化活性,并借助高效液相色谱联用多角度激光散射(HPSEC MALLS RI)技术和红外光谱分析多糖初步结构。结果表明:最佳提取条件为缓冻时间16 h、微波功率260 W、液料比53 mL/g、提取时间8 min,在最佳提取条件下,PW 1的得率为11.87%。在4种抗氧化活性试验中,PW 1表现的抗氧化活性比PW 2更强。初步结构分析发现PW 1与PW 2分别是由4个主色谱峰和3个主色谱峰构成,并以α糖苷键连接为主的吡喃型糖,重均分子量均为1.018×10^(6)~14.980×10^(6)g/mol。
The extraction process of Dictyophora indusiata polysaccharides by slow freezing-microwave assisted method was studied.The factors of slow freezing time,microwave power,liquid-solid ratio,extraction time was investigated,and then the response surface design was used to obtain the optimal extraction process based on the single-factor experiment.The antioxidant activity of 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-bis(3-ethylbenzothiazoline-6-sulfonic acid)diamimium salt(ABTS)and hydroxyl radical scavenging and total reducing capacity were compared with the polysaccharide(PW-1)extracted by slow freezing-microwave assisted method and the polysaccharide(PW-2)obtained by microwave assisted method.The preliminary structure of polysaccharides was analyzed by high-performance liquid chromatography multi-angle laser scattering(HPSEC-MALLS-RI)technology and infrared spectroscopy.Results showed that the optimum extraction conditions for the 11.87%extraction yield of polysaccharide(PW-1)was slow freezing time of 16 h,microwave power of 260 W,liquid-solid ratio of 53 mL/g,and extraction time of 8 min.PW-1 exhibited stronger antioxidant activity than PW-2 in four tests of antioxidant activity.PW-1 and PW-2 were composed of four main chromatographic peaks,and three main chromatographic peaks respectively.The molecular weights of pyranose connected byα-glycosidic bonds ranged from 1.018×10^(6) to 14.980×10^(6) g/mol.