文献类型：期刊文献

中文题名：南海水域不同深度非光合微生物的固碳潜能及其对不同电子供体的响应

英文题名：Potential Carbon Fixation Capability of Non-photosynthetic Microbial Community at Different Depth of the South China Sea and Its Response to Different Electron Donors

作者：方峰[1];王磊[2];席雪飞[2];胡佳俊[2];付小花[2];陆兵[1];徐殿胜[1]

机构：[1]华东理工大学生物工程学院生物反应器工程国家重点实验室,上海200237;[2]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

年份：2015

卷号：36

期号：5

起止页码：1550

中文期刊名：环境科学

外文期刊名：Environmental Science

收录：MEDLINE(收录号：26314099);CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；PubMed;

基金：国家自然科学基金项目(21177093;21307093);国家高技术研究发展计划(863)项目(2012AA050101);高等学校博士学科点专项科研基金项目(20130072110025);中国博士后科学基金项目(2013M531220;2014T70430)

语种：中文

中文关键词：南海水域;固碳潜力;基因丰度;海水深度;影响因素

外文关键词：South China Sea;;potential carbon fixation capability;;gene abundance;;depth of the sea;;affecting factors

摘要：通过对南海水域多个区域不同深度的海水分别以H2、Na2S2O3、Na NO2为电子供体经过一定时间的驯化培养后,测定其中非光合微生物的固碳潜力,并统计南海水域不同深度非光合微生物在不同电子供体条件下固碳潜力的差异性,最后结合不同深度海水的主要固碳基因丰度差异,分析南海水域不同深度海洋非光合微生物在不同电子供体条件下固碳潜能差异的原因.结果发现以Na NO2为电子供体时,海洋非光合微生物固碳能力普遍较低,各深度之间没有显著差异;以H2为电子供体时,表层海水中的非光合微生物的固碳潜力显著高于深层海水中的;而以Na2S2O3为电子供体时,深层海水中的非光合微生物的固碳潜力显著高于表层海水中的.基因分析结果表明,固碳基因cbb L在表层海水中的丰度高于深层海水,而cbb M基因在深层海水中的丰度高于表层海水.硫细菌大多以拥有cbb M基因为主,而氢细菌大多以拥有cbb L基因为主.因此不同海洋深度非光合微生物对不同电子供体响应的差异性可能和优势菌群结构的差异有关.海洋表层和深层溶解氧、无机碳含量的差异是导致菌群结构差异,乃至固碳潜力差异的重要原因.
The seawater samples collected from many different areas with different depth in the South China Sea were cultivated using different electron donors respectively. And the variation in the potential carbon fixation capability( PCFC) of non-photosynthetic microbial community( NPMC) in seawater with different depth was determined after a cycle of cultivation through the statistic analysis.In addition,the cause for the variation was clarified through analyzing key gene abundance regarding CO2 fixation and characteristics of seawater with different depth. The result showed that the PCFCs of NPMC in seawater with different depth were generally low and had no significant difference when using Na NO2 as the electron donor. The PCFC of NPMC in surface seawater was higher than that in deep seawater when using H2 as the electron donor,on the contrary,the PCFC of NPMC in deep seawater was higher than that in surface seawater when using Na2S2O3 as the electron donor. The abundance of the main CO2 fixation gene cbb L in surface seawater was higher than that in deep seawater while the cbb M gene abundance in deep seawater was higher than that in surface seawater. Most hydrogenoxidizing bacteria had the cbb L gene,and most sulfur bacteria had the cbb M gene. The tendency of seawater cbb L / cbb M gene abundance with the change of depth revealed that there were different kinds of bacteria accounting for the majority in NPMC fixing CO2 at different depth of ocean,which led to different response of PCFC of NPMC at different depth of the sea to different electron donors.The distributions of dissolved oxygen and inorganic carbon concentration with the change of the depth of the sea might be an important reason leading to the difference of NPMC structure and even the difference of PCFC at different depth of the sea.