文献类型：期刊文献

英文题名：Fluorescence lifetime clocks quantify senescence and aging

作者：Yan, Chenxu[1,2];Liu, Caiqi[1,2];Liu, Bofang[3];Zheng, Qiaoqiao[4];Zhao, Xingyuan[1,2];Lu, Sirui[1,2];Fu, Xiaoqing[1,2];Li, Wennan[1,2];Li, Juan[1,2];Li, Dan[1,2];Yao, Yongkang[1,2];Zhang, Yutao[1,2];Zhu, Huanhu[3];Shi, Ping[4];Zhu, Wei-Hong[1,2,5];Guo, Zhiqian[1,2,4]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem,Key Lab Adv Mat, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem,Sch Che, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Sch Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem,Joint, Shanghai, Peoples R China;[3]ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, Ctr Photosensit Chem Engn, Shanghai, Peoples R China

年份：2025

卷号：5

期号：12

起止页码：2532

外文期刊名：NATURE AGING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001608984800001)】；

基金：This work was supported by National Key Research and Development Program (2023YFA1802000 to Z.G.), NSFC/China (22225805, 32121005, 32394001, T2488302 and 22378122 to Z.G., Z.G., Z.G., W.-H.Z. and C.Y., respectively), Shanghai Science and Technology Innovation Action Plan (No. 23J21901600 to Z.G.), Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28 to Z.G.), and Science and Technology Commission of Shanghai Municipality (grant No. 24DX1400200 to Z.G.). We thank M. Zhu (Renji Hospital, Shanghai Jiao Tong University) for carrying out volunteer in different age recruitment and sample collection, and A. Shao (Jiangnan University), M. Zhao, J. Huang, Y. Zhang, J. Yang, W. Zhang and X. Zhao (East China University of Science and Technology) for culturing cells.

语种：英文

摘要：Epigenetic and omics-based clocks have provided invaluable tools to quantify aging, yet these clocks do not provide direct readouts of aging in real-time in living systems. As methylation changes in nucleolar ribosomal DNA are reliably associated with aging and cellular senescence, we hypothesized that shifts in rRNA species could be leveraged to generate image-based clocks using selective dyes. Here we engineer sensitive and photostable hybrid polymethine dyes selective for rRNA. We present a fluorescence lifetime imaging strategy to visually quantify age- and cellular senescence-dependent nucleolar RNA changes that bypasses requirements for extensive sample preparation such as DNA isolation and enables in vivo, real-time age quantification. We demonstrate resolution through cellular to organismal scales and demonstrate translatability by generating clocks from cells and tissues, as well as Caenorhabditis elegans, mice and human samples. Our fluorescence lifetime imaging strategy thus enables in vivo measurements of aging and senescence and expands the toolbox for aging biology research and translation.