文献类型：会议论文

英文题名：Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study

作者：Zhang, Min[2];Ignatova, Svetlana[1];Hu, Ping[3];Liang, Qionglin[4];Wang, Yiming[4];Luo, Guoan[2,4];Jun, Frank Wu[5];Sutherland, Ian[1]

机构：[1]Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England;[2]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Tsinghua Univ, Inst Biomed, Beijing 100084, Peoples R China;[5]XiangCam TCM Res Ctr Ltd, Cambridge CB22 3AT, England

会议论文集：6th International Conference on Countercurrent Chromatography

会议日期：JUL 28-30, 2010

会议地点：Univ Lyon, La Doua Campus, Lyon, FRANCE

主办单位：Univ Lyon, La Doua Campus

语种：英文

外文关键词：High-performance counter-current chromatography; Green purification process; Tanshinone IIA; Crypotanshinone; Salvia miltiorrhiza Bunge

摘要：A strategy for the development of a green process using counter-current chromatography technology is presented in this paper. The strategy began with solvent system selection, followed by linear scale-up from an analytical to a preparative process with optimized operating parameters. A two-stage separation using a multi-injection method was performed with a solvent system of hexane-dichloromethane-methanol-water (4:0.75:4:1) for the 1st stage and a hexane-ethanol-water (4:2:2) for the 2nd stage. A 1918 mg of tanshinone IIA was purified, with a 97% purity and 34.4% recovery and a 276.7 mg of cryptotanshinone was separated, with a 95% purity and 31.8% recovery from 2.1 g of crude extract. Process parameters (throughput, efficiency, environmental risk factor and general process evaluation) and mass factors (mass intensity, separation mass efficiency and greenness) of a target were developed for monitoring of the counter-current chromatography process. (C) 2011 Elsevier B.V. All rights reserved.