文献类型：期刊文献

英文题名：Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica

作者：Cao, Xuan[1];Wei, Liu-Jing[1];Lin, Jia-Yu[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：245

起止页码：1641

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20172903956703);WOS:【SCI-EXPANDED(收录号:WOS:000413038300042)】；

基金：This work was financially supported by National Natural Science Foundation of China (21576089).

语种：英文

外文关键词：Linalool; Yarrowia lipolytica; Linalool synthase; MVA pathway

摘要：In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production. A maximum linalool titre of 6.96 +/- 0.29 mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts. (C) 2017 Elsevier Ltd. All rights reserved.