文献类型：期刊文献

英文题名：Biosynthesis of Medium-Chain ω-Hydroxy Fatty Acids by AlkBGT of Pseudomonas putida GPo1 With Native FadL in Engineered Escherichia coli

作者：He, Qiaofei[1];Bennett, George N.[5];San, Ka-Yu[4,5];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, Shanghai, Peoples R China;[4]Rice Univ, Dept Bioengn, Houston, TX USA;[5]Rice Univ, Dept Chem & Bimol Engn, Houston, TX USA

年份：2019

卷号：7

期号：OCT

外文期刊名：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

收录：;EI(收录号：20194607672779);WOS:【SCI-EXPANDED(收录号:WOS:000497402000003)】；

基金：This study was supported by the National Key Research and Development Program of China (Grant No. 2017YFB0309302), the National Natural Science Foundation of China (Grant No. 21776083), the Fok Ying-Tong Education Foundation, China (Grant No. 161017), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014), and the 111 Project (B18022), the Science and Technology Commission of Shanghai Municipality (Grant No. 17JC1404800). This study was partially supported by the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Escherichia coli; AlkBGT; medium-chain fatty acids; omega-hydroxy fatty acids; FadL

摘要：Hydroxy fatty acids (HFAs) are valuable compounds that are widely used in medical, cosmetic and food fields. Production of omega-HFAs via bioconversion by engineered Escherichia coli has received a lot of attention because this process is environmentally friendly. In this study, a whole-cell bio-catalysis strategy was established to synthesize medium-chain omega-HFAs based on the AlkBGT hydroxylation system from Pseudomonas putida GPo1. The effects of blocking the beta-oxidation of fatty acids (FAs) and enhancing the transportation of FAs on omega-HFAs bio-production were also investigated. When fadE and fadD were deleted, the consumption of decanoic acid decreased, and the yield of omega-hydroxydecanoic acid was enhanced remarkably. Additionally, the co-expression of the FA transporter protein, FadL, played an important role in increasing the conversion rate of omega-hydroxydecanoic acid. As a result, the concentration and yield of omega-hydroxydecanoic acid in NH03(pBGT-fadL) increased to 309 mg/L and 0.86 mol/mol, respectively. This whole-cell bio-catalysis system was further applied to the biosynthesis of omega-hydroxyoctanoic acid and omega-hydroxydodecanoic acid using octanoic acid and dodecanoic acid as substrates, respectively. The concentrations of omega-hydroxyoctanoic acid and omega-hydroxydodecanoic acid reached 275.48 and 249.03 mg/L, with yields of 0.63 and 0.56 mol/mol, respectively. This study demonstrated that the overexpression of AlkBGT coupled with native FadL is an efficient strategy to synthesize medium-chain omega-HFAs from medium-chain FAs in fadE and fadD mutant E. coli strains.