文献类型：期刊文献

中文题名：双罗丹明类衍生物荧光探针的合成及对Cu^(2+)的识别

英文题名：Synthesis of a Birhodamine-based Fluorescent Probe and Recognition Properties for Cu^(2+)

作者：袁跃华[1];朱永军[1];殷强锋[2];冯锋[1];王海雁[1];田茂忠[1]

机构：[1]山西大同大学化学与环境工程学院,山西大同037009;[2]华东理工大学化工学院,上海200237

年份：2018

卷号：34

期号：3

起止页码：327

中文期刊名：分析科学学报

外文期刊名：Journal of Analytical Science

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(No.21175085;21375083);大同市基础研究计划项目(No.2017126)

语种：中文

中文关键词：荧光探针;罗丹明6G;铜离子

外文关键词：Fluorescence probe;Rhodamine 6G;Cu^2＋

摘要：设计并合成了一种可检测Cu^(2+)的新双罗丹明类衍生物荧光增强型分子探针(RG1),并用核磁和高分辨质谱(HRMS)对其结构进行了表征。通过紫外-可见光谱和荧光光谱研究了该荧光分子探针对Cu^(2+)的识别性能,结果表明:在体积比为1∶1的乙腈-水(10mmol/L Tris-HCl,pH=7.2)溶液体系中,探针RG1本身无色且荧光很弱,加入Cu^(2+)后,探针在553nm处出现强的荧光发射峰,溶液颜色从无色变为粉红色,对Cu^(2+)表现出好的选择性。此外,该探针可在较宽的pH范围(5~10)内直接检测Cu^(2+)的浓度。结果表明,Cu^(2+)浓度在3.0×10^(-6)~1.5×10^(-5)mol/L范围内与探针的荧光强度呈良好的线性关系,Cu^(2+)检出限为3.31×10^(-7)mol/L。同时对河水进行加标回收实验,得到了良好的回收率。
A novel birhodamine-based fluorescent enhancement molecular probe（RG1）for the detection of Cu^2＋ was designed and synthesized.Its structure was characterized by ^1H NMR,^13C NMR and high resolution mass spectrometer（HRMS）.The recognition properties of this fluorescent molecular probe for Cu^2＋were studied by UV-Vis spectra and fluorescence spectra.The results showed that in acetonitrile-water（1∶1,V/V）solution the probe was colorless and the fluorescence intensity was very weak.Upon the addition of Cu^2＋,a new fluorescence emission peak at 553 nm appeared with increasing intensity evidently.The color of the solution changed from colorless to pink.Probe RG1 showed good selectivity to Cu^2＋.In addition,the probe could detect the concentration of Cu^2＋ directly in a wide range of pH（5-10）.The concentration of Cu^2＋ was in good linearity with the fluorescence intensity of the probe RG1 in the range of 3.0×10^-6-1.5×10^-5mol/L with a detection limit of 3.31×10^-7mol/L.At the same time,the standard recovery experiments of river sample were carried out with satisfactory result.