文献类型：期刊文献

英文题名：Rapid Analysis for Staphylococcus aureus via Microchip Capillary Electrophoresis

作者：Chen, Jin[1];Sun, Yu[1];Peng, Xiaogai[1];Ni, Yi[2];Wang, Fengchao[1];Dou, Xiaoming[2,3]

机构：[1]Shanghai Inst Technol, Coll Sci, 100 Haiquan Rd, Shanghai 201418, Peoples R China;[2]East China Univ Sci & Technol, Inst Photon & Biomed, Grad Sch Sci, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Osaka Univ, Dept Appl Phys, Grad Sch Engn, Suita, Osaka 5650871, Japan

年份：2021

卷号：21

期号：4

起止页码：1

外文期刊名：SENSORS

收录：;EI(收录号：20210709924840);WOS:【SCI-EXPANDED(收录号:WOS:000624676700001)】；

基金：The project was sponsored by Shanghai Sailing Program, China (No.18YF1422500), Research start-up project of Shanghai Institute of Technology (No.YJ2018-9), and the Collaborative innovation project of Shanghai Institute of Technology (No. XTCX2020-12).

语种：英文

外文关键词：Staphylococcus aureus; nuc gene; microchip capillary electrophoresis; space domain internal standard method

摘要：Staphylococcus aureus (S. aureus) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of S. aureus, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of S. aureus nuc gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/mu L, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis.