文献类型：期刊文献

英文题名：Hypercrosslinked strong cation-exchange polymers for selective extraction of serum purine metabolites associated with gout

作者：Xu, Yating[1];Liu, Ju[2];Zhang, Hongyang[1];Jiang, Min[2];Cao, Lingling[3];Zhang, Min[4,5];Sun, Wei[1];Ruan, Shengli[1];Hu, Ping[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Nanchang Univ, Affiliated Jiujiang Hosp, Jiujiang Peoples Hosp 1, Dept Rheumatism, Jiujiang 332000, Peoples R China;[3]Nanchang Univ, Affiliated Jiujiang Hosp, Jiujiang Peoples Hosp 1, Dept Internal Med, Jiujiang 332000, Peoples R China;[4]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[5]E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr TCM, Shanghai 200237, Peoples R China

年份：2016

卷号：151

起止页码：172

外文期刊名：TALANTA

收录：;EI(收录号：20160501881594);WOS:【SCI-EXPANDED(收录号:WOS:000372761900022)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81202493, 81273481, 81460170), the Fundamental Research Funds from the Central Universities, the National Science and Technology Major Project for Significant New Drugs Development (No. 2013ZX09507005), and the Shanghai Natural Science Foundation (No. 15ZR1409400).

语种：英文

外文关键词：Hypercrosslinked strong cation-exchange polymers; Solid-phase extraction; Basic purine metabolites; Human serum; Gout

摘要：In this study, hypercrosslinked strong cation-exchange polymer resins (HXLPP-SCX) were synthesized and employed as selective sorbents for the solid-phase extraction (SPE) of basic purine metabolites associated with gout. The HXLPP-SCX material was prepared based on hypercrosslinking reactions and sulfonated with concentrated H2SO4. This synthetic procedure is facile and efficient without using highly toxic reagent. The resulting resins were characterized in the form of monodisperse microspheres (mean diameters of 3-5 mu m) with narrow pore size (2.1 nm) and relatively high specific surface areas (801 m(2)/g). The polymers also possess high ion-exchange capacity (IEC, 2.22 mmol/g) and good adsorption and selectivity performances for basic compounds. The resins used as SPE sorbents permit the selective enrichment of three pivotal purine metabolites (hypoxanthine, xanthine and inosine) in human serum followed by HPLC analysis. Method validation including linearity range, sensitivity, accuracy and reproducibility were evaluated. This method was exemplarily applied in the analysis of serum purines in gout patients and healthy controls. The present results demonstrate a promising potential of this HXLPP-SCX material for the clinical sample pretreatment. (C) 2016 Elsevier B.V. All rights reserved.