文献类型：期刊文献

英文题名：Simultaneous observation of the spatial and temporal dynamics of single enzymatic catalysis using a solid-state nanopore

作者：Yu, Ru-Jia[1,2,3];Li, Qiao[3];Liu, Shao-Chuang[1,3];Ma, Hui[1];Ying, Yi-Lun[1,2];Long, Yi-Tao[1]

机构：[1]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China;[2]Nanjing Univ, Chem & Biomed Innovat Ctr, Nanjing 210023, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：15

期号：16

起止页码：7261

外文期刊名：NANOSCALE

收录：;EI(收录号：20231613901463);WOS:【SCI-EXPANDED(收录号:WOS:000968106100001)】；

基金：This research was supported by the National Natural Science Foundation of China (Grant No. 22090051, 22276089, 21834001 and 22104052).

语种：英文

外文关键词：Glucose - Glucose oxidase - Glucose sensors - Molecules

摘要：We developed a bipolar SiNx nanopore for the observation of single-molecule heterogeneous enzymatic dynamics. Single glucose oxidase was immobilized inside the nanopore and its electrocatalytic behaviour was real-time monitored via continuous recording of ionic flux amplification. The temporal heterogeneity in enzymatic properties and its spatial dynamic orientations were observed simultaneously, and these two properties were found to be closely correlated. We anticipate that this method offers new perspectives on the correlation of protein structure and function at the single-molecule level.