文献类型：期刊文献

英文题名：Mass Transport Effect on Graphene Based Enzyme Electrochemical Biosensor for Oxalic Acid Detection

作者：Zhang, Yan[1];Wu, Congyu[2];Zhang, Jingyan[3];Guo, Shouwu[2]

机构：[1]Shanghai Univ Engn Sci, Sch Mat Engn, Shanghai 201620, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Elect Engn, Shanghai 200240, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：164

期号：2

起止页码：B29

外文期刊名：JOURNAL OF THE ELECTROCHEMICAL SOCIETY

收录：;EI(收录号：20175104553227);WOS:【SCI-EXPANDED(收录号:WOS:000397850800007)】；

基金：This work was supported by the National "973 Program" of China (grant Nos. 2014CB260411, 2015CB931801), National Natural Science Foundation of China (grant Nos. 11374205, 51602192), the State Key Laboratory of Bioreactor Engineering (grant No. 2060204), Shanghai "Sailing Program" (grant No. 14YF1409500), research fund for the doctoral program of Shanghai University of Engineering Science (grant No. 2014), and the Project of Shanghai Universities Young Teacher Training Scheme (grant No. ZZgcd14011).

语种：英文

外文关键词：Enzyme electrodes - Diffusion - Electrochemical biosensors - Electrochemical electrodes - Electron transitions - Oxalic acid

摘要：Graphene has been widely used to construct enzyme biosensors, with most research focused on improving electron transfer. While most of electrochemical process on graphene-modified electrode has been demonstrated to be a diffusion-controlled, the restacking of graphene has resulted in nonefficient mass transport. Herein, oxalate oxidase (OxOx) immobilized on chemically reduced graphene oxide (CRGO) can suppress the aggregation of CRGO and exhibit significantly improved mass transport and can be used as biosensor with excellent sensitivity to oxalic acid. More importantly, the amount of CRGO has a significant effect on electron transfer and the mass transport. By adjusting amount of CRGO, we demonstrate that the electron-transfer rate and mass-diffusion efficiency have a synergistic effect on the electrochemical performance of biosensors. Improving the mass diffusion rate can greatly enhance the electrochemical properties of a graphene based biosensor; therefore, the efficient mass transport on graphene based electrodes should receive more attention. A detailed understanding of this could be further applied to other graphene-based enzyme biosensors. (C) 2016 The Electrochemical Society. All rights reserved.