文献类型：期刊文献

英文题名：The novel repressor Rce2 competes with Ace3 to regulate cellulase gene expression in the filamentous fungus Trichoderma reesei

作者：Chen, Yumeng[1];Wang, Wei[1];Liu, Pei[1];Lin, Aibo[1];Fan, Xingjia[1];Wu, Chuan[1];Li, Ni[1];Wei, Liujing[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：116

期号：5

起止页码：1298

外文期刊名：MOLECULAR MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000708690900001)】；

基金：Shanghai Agriculture Applied Technology Development Program, China, Grant/Award Number: 2021-02-08-00-12-F00758; National Natural Science Foundation of China, Grant/Award Number: 32000050; China Postdoctoral Science Foundation, Grant/Award Number: 2019M661402

语种：英文

外文关键词：cellulase; gene regulation; repressor; transcription factor; Trichoderma reesei

摘要：The filamentous fungus Trichoderma reesei is widely used for industrial cellulase production. In T. reesei, cellulase gene expression is tightly controlled by a regulatory network involving multiple transcription factors. Here, we isolated a novel protein, Rce2, using a pull-down assay and mass spectrometry analysis, from a partial carbon catabolite de-repression mutant, T. reesei Rut-C30, cultured under glucose-repressing conditions. Deletion and overexpression of Rce2 in T. reesei wild-type QM6a and mutant Rut-C30 revealed that Rce2 acts as a repressor of cellulase gene expression. DNase I footprinting assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation assays revealed that Rce2 was located in the nucleus and bound to the consensus sequences 5 '-(T/A)NNNNCCG-3 ' and 5 '-CGGNNNN(T/A)-3 ' in the promoters of cellulase-related genes to repress their transcription. Additionally, Rce2 antagonized Ace3 binding to the cbh1 promoter to repress its transcription. However, Rce2 was not involved in Cre1-mediated carbon catabolite repression. These results demonstrate the mechanism through which Rce2 represses the expression of cellulase genes and provide novel insights into the regulatory system of cellulases and methods that can be used for the regulation of gene expression in T. reesei.