文献类型：期刊文献

英文题名：Engineering DNA aptamers for novel analytical and biomedical applications

作者：You, Mingxu[2,3];Chen, Yan[2,3,4];Peng, Lu[2,3];Han, Da[2,3];Yin, Bincheng[1,2,3];Ye, Bangce[1];Tan, Weihong[2,3,4]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China;[2]Univ Florida, Dept Chem & Physiol, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA;[3]Univ Florida, Shands Canc Ctr, Gainesville, FL 32611 USA;[4]Hunan Univ, Coll Chem & Chem Engn, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

年份：2011

卷号：2

期号：6

起止页码：1003

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20112314030580);WOS:【SCI-EXPANDED(收录号:WOS:000290629900001)】；

基金：The authors would like to thank Dr Kathryn R. Williams for manuscript review. We acknowledge NIH and NSF for the funding this project. This work was supported by the National Key Scientific Program of China (2011CB911000) and China National Grand Program on Key Infectious Disease (2009ZX10004-312). This work was also supported by Grant 20627005 from the National Natural Science Foundation, the National Special Fund for SKLBE (2060204).

语种：英文

外文关键词：Binding energy - Chemical modification - Medical applications - Biochemistry - Nucleic acids

摘要：As an alternative to antibodies, aptamers have shown promising applications in diagnostics and therapeutics. However, different from antibodies, the chemical nature of nucleic acids allows easy synthesis and modification of aptamers. As a result, there are various feasible ways to engineer aptamers with extended bioavailability (e. g., stability and binding affinity in complex environments), regulating ability, and multi-functional properties. In this review, recent advances in rational design and novel functionalization of aptamers, especially DNA aptamers, is described. The broad spectrum of ways for aptamer engineering and applications is paving the way for the future evolution of bioanalytical and biomedical developments.