文献类型：期刊文献

英文题名：Catalytic resolution of (RS)-HMPC acetate by immobilized cells of Acinetobacter sp CGMCC 0789 in a medium with organic cosolvent

作者：Chen, Y; Xu, JH; Pan, J; Xu, Y; Shi, JB

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China

年份：2004

卷号：30

期号：5-6

起止页码：203

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000223552000004)】；

语种：英文

外文关键词：Acinetobacter sp esterase; enantioselectivity; enzymatic resolution; 4-hydroxy-3-methyl-2-(2 '-propenyl)-2-cyclopentenone; immobilized cells; isopropanol

摘要：Kinetic resolution of a chiral alcohol, 4-hydroxy-3-methyl-2-(2'-propenyl)-2-cyclopentenone (HMPC), a key intermediate for the production of prallethrin insecticides, was successfully carried out by enantioselective hydrolysis of (RS)-HMPC acetate using calcium alginate gel-entrapped cells of a newly isolated esterase-producing bacterium Acinetobacter sp. CGMCC 0789. When the effect of different cosolvents was investigated, it was found that isopropanol could markedly enhance the activity and enantioselectivity of the immobilized cells. The optimum concentration of isopropanol was 10% (v/v) where immobilized cells still showed good operational stability. After 10 cycles of reaction, no significant decrease in the enzyme activity was observed. The catalytic specificity constants (V-max/K-m) for both enantiomers of the substrate were determined with partially purified enzyme, giving 0.0184 and 0.671 h(-1) for the (S)- and (R)-ester, respectively. (C) 2004 Published by Elsevier B.V.