文献类型：期刊文献

英文题名：Synthesis and Antibacterial Activities of Antibacterial Peptides with a Spiropyran Fluorescence Probe

作者：Chen, Lei;Zhu, Yu;Yang, Danling;Zou, Rongfeng;Wu, Junchen[1];Tian, He

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：4

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000343989600011)】；

基金：We thank the National Basic Research 973 Program (2013CB733700), the Fundamental Research Funds for the Central Universities (WJ1213007) and the Innovation Program of Shanghai Municipal Education Commission (J100-2-13104) for financial support.

语种：英文

摘要：In this report, antibacterial peptides1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection.