文献类型：期刊文献

英文题名：Novel chemically synthesized hydroxyl-containing jasmonates as powerful inducing signals for plant secondary metabolism

作者：Qian, Zhi-Gang[1]; Zhao, Zhen-Jiang[2]; Xu, Yufang[2]; Qian, Xuhong[2,3]; Zhong, Jian-Jiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Pesticides & Pharmaceut, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[3]Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China

年份：2004

卷号：86

期号：7

起止页码：809

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：2004268228284);WOS:【SCI-EXPANDED(收录号:WOS:000221776100010)】；

语种：英文

外文关键词：plant cell culture; Taxus chinensis; synthetic jasmonate derivatives; inducing signal; secondary metabolism; plant defense response

摘要：Novel hydroxyl-containing jasmonate derivatives were chemically synthesized and evaluated by bioassay as potential elicitors for stimulating the biosynthesis of plant secondary metabolites. A suspension culture of Taxus chinensis, which produces a bioactive taxoid, taxuyunnanine C (Tc), was taken as a model plant cell system. Experiments on the timing of addition of jasmonates and dose response indicated that day 7 and 100 muM was the optimal elicitation time and concentration, respectively, for both cell growth and Tc accumulation. Tc accumulation was increased more in the presence of hovel hydroxyl-containing jasmonates compared to that with methyljasmonate (MJA) addition. For example, addition of 100 muM 2,3-dihydroxypropyl jasmonate on day 7 led to a very high Tc content of 47.2 +/- 0.5 mg/g (at day 21), whereas the Tc content was 29.2 +/- 0.6 mg/g (on the same day) with addition of 100 muM MJA. Quantitative structure -activity analysis of various jasmonates suggests that the optimal lipophilicity and the number of hydroxyl groups may be two important factors affecting their elicitation activity. In addition, the jasmonate elicitors were found to induce plant defense responses, including oxidative burst and activation Of L-phenylalanine ammonia lyase (PAL). Interestingly, a higher level of H2O2 production and PAL activity was detected with elicitation by the synthesized jasmonates compared with that by MJA, which corresponded well to the superior stimulating activity in the former. This work indicates that the newly synthesized hydroxyl-containing jasmonates can act as powerful inducing signals for secondary metabolite biosynthesis in plant cell cultures. (C) 2004 Wiley Periodicals, Inc.