文献类型：期刊文献

英文题名：Improvement of expression level and bioactivity of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) by a novel zinc ion feeding strategy

作者：Sun, Ai-You[1]; Shen, Ya-Ling[1]; Yin, Ji-Cheng[1]; Zhang, Hong[1]; Tang, Ya-Nan[1]; Wei, Dong-Zhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：28

期号：15

起止页码：1215

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20063010023032);WOS:【SCI-EXPANDED(收录号:WOS:000239085500012)】；

语种：英文

外文关键词：apoptosis; Escherichia coli; tumor necrosis factor; zinc ion; feeding strategy

摘要：Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL) is a new member of the TNF superfamily. In this work, the key role of Zn2+ in high-level expression of soluble TRAIL was confirmed. The yield of soluble TRAIL reached 1.6 g l(-1) using a novel, two-stage Zn2+ feeding strategy, and the accumulation of TRAIL inclusion bodies decreased. Furthermore, the purified TRAIL showed stronger cytotoxicity activity against human pancreatic 1990 tumor cells as the molar ratio of Zn2+ to TRAIL monomer was 2 in purified TRAIL solution.