文献类型：期刊文献

英文题名：Population genetic characterisation of dominant Cryptosporidium parvum subtype IIaA15G2R1

作者：Feng, Yaoyu[1];Torres, Eucaris[2];Li, Na[1,2];Wang, Lin[1,2];Bowman, Dwight[3];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA;[3]Cornell Univ, Dept Microbiol & Immunol, Coll Vet Med, Ithaca, NY 14853 USA

年份：2013

卷号：43

期号：14

起止页码：1141

外文期刊名：INTERNATIONAL JOURNAL FOR PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000328924700006)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005 and 31110103901.), National Special Fund for State Key Laboratory of Bioreactor Engineering, China (No. 2060204), Fundamental Research Funds for the Central Universities, China, and Centers for Disease Control and Prevention, USA. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cryptosporidium; Cryptosporidiosis; Cryptosporidium parvum; IIaA15G2R1; Virulence; Population genetics

摘要：The subtype IIaA15G2R1 at the 60 kDa glycoprotein (gp60) gene locus is the most dominant Cryptosporidium parvum infecting dairy cattle and humans in industrialised nations. The reasons for its high transmissibility are not clear, and it remains to be determined whether this subtype represents a homogeneous parasite population. In this study, we sequence-characterised 26 IIaA15G2R subtype specimens and 26 non-IIaA15G2R subtype specimens from the United States, Canada, United Kingdom and Spain at seven other known polymorphic loci, including CP47, CP56, DZ-HRGP, MSC6-5, MSC6-7, RPGR and ZPT. Extensive heterogeneity within IIaA15G2R1 and discordance in typing results between gp60 and other genetic markers were observed. Results of inter-locus and intra-ZPT linkage disequilibrium and recombination analyses indicated that the heterogeneity within IIaA15G2R1 and discordance in typing results among genetic loci were largely due to the occurrence of genetic recombination, mostly within the gp60 subtype IIaA15G2R1. Although there was no clear population diversion between IIaA15G2R and non-IIaA15G2R subtypes, results of STRUCTURE and F-ST analyses suggested the presence of at least two subpopulations; subpopulation 1 had an epidemic population structure and was widely distributed, whereas subpopulation 2 had a clonal population structure and consisted of geographically segregated multilocus subtypes. Genetic recombination between epidemic and geographically segregated C. parvum populations appeared to be a driving force in the emergence of a hyper-transmissible IIaA15G2R1 subtype. Genetic recombination was observed even between the zoonotic IIa subtype family and anthroponotic subtype family IIc at CP56, MSC6-7 and ZPT. Thus, the IIaA15G2R1 subtype at gp60 is likely a fitness marker for C. parvum and the wide spread of IIaA15G2R1 subtype around the world is probably independent of the sequence characteristics at other genetic loci. (C) 2013 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.