文献类型：期刊文献

中文题名：灵芝子实体中不同极性的三萜体外抗肿瘤及抗炎活性比较

英文题名：In vitro antitumor and anti-inflammatory activities of triterpenoids with different polarity in the fruiting bodies of Ganoderma lingzhi

作者：郝萌萌[1,2];王金艳[2];冯娜[2];张劲松[2];唐庆九[2];韩伟[1]

机构：[1]华东理工大学药学院上海市新药设计重点实验室,上海200237;[2]上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室,上海201403

年份：2019

卷号：38

期号：6

起止页码：917

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-3-3号]~~

语种：中文

中文关键词：总三萜;中等极性三萜;低极性三萜;抗肿瘤;抗炎

外文关键词：total triterpenoids;medium polar triterpenoids;low polar triterpenoids;antitumor activity;anti-inflammatory activity

摘要：为了解灵芝中不同极性三萜活性的差异,运用大孔树脂将灵芝总三萜根据极性大小进行分段,采用高效液相法分析各极性部分的HPLC指纹图谱和三萜含量,并比较其对不同肿瘤细胞增殖的抑制作用和抗炎活性。结果显示D-101大孔树脂可以有效地将灵芝总三萜分为中等极性和低极性两部分,两部分的三萜含量分别为(279.00±2.90)mg/g、(94.52±2.03)mg/g。其中,低极性三萜的体外抗肿瘤活性明显强于中等极性三萜,其对K562、SW620、L1210细胞增殖抑制的IC50值分别为(74.12±1.94)μg/mL、(121.45±2.13)μg/mL、(13.52±1.13)μg/mL。另外,低极性三萜对RAW264.7细胞呼吸爆发的抑制作用也明显强于中等极性三萜。进一步对低极性三萜和中等极性三萜单体的抗炎活性进行比较,发现低极性三萜灵芝烯酸F、灵芝酸DM、灵芝醇B对RAW264.7细胞呼吸爆发的抑制作用明显强于极性较高的灵芝酸C2、灵芝酸A。该研究阐明了灵芝子实体中不同极性三萜部位的抗肿瘤及抗炎活性并不相同,为将来新药开发和灵芝质量标准的改进建立了基础。
To investigate the activities of triterpenoids with different polarity from the fruiting body of Ganoderma lingzhi,the total triterpenoid extract was separated into different polarity extracts by macroporous resin.Fingerprints and the content of triterpenoids were analyzed by HPLC.Anti-tumor and anti-inflammatory activities of different polarity extracts were compared.The results showed that the total triterpenoids can be separated by D-101 macroporous resin effectively into two polar parts,medium polar triterpenoid and low polar triterpenoid,with the content of(279.00±2.90)mg/g and(94.52±2.03)mg/g respectively.The anti-tumor activity of low polar triterpenoid is much stronger than that of medium polar triterpenoid.The IC50 of inhibition to K562 cells,SW620 cells,and L1210 cells are(74.12±1.94)μg/mL,(121.45±2.13)μg/mL and(13.52±1.13)μg/mL,respectively.The suppression effect of low polar triterpenoid on PMA-stimulated respiratory burst in RAW264.7 macrophages is also obviously stronger than that of medium polar triterpenoid.The comparison of the suppression effect between different triterpene monomers on PMA-stimulated respiratory burst in RAW264.7 macrophages shows that ganoderenic acid F,ganoderic acid DM and ganoderol B,with low polarity,have stronger anti-inflammatory activity than ganoderic acid C2 and ganoderic acid A with medium polarity.It is proved that the antitumor and anti-inflammatory activities in vitro of triterpenoids with different polarity from the fruiting bodies of Ganoderma lingzhi are different significantly.This study provides reference for improving quality standards of the Ganoderma lingzhi products.