文献类型：期刊文献

英文题名：Genome-Inspired Chemical Exploration of Marine FungusAspergillus fumigatusMF071

作者：Han, Jianying[1,2];Liu, Miaomiao[1];Jenkins, Ian D.[1];Liu, Xueting[3];Zhang, Lixin[2,3];Quinn, Ronald J.[1];Feng, Yunjiang[1]

机构：[1]Griffith Univ, Griffith Inst Drug Discovery, Brisbane, Qld 4111, Australia;[2]Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：18

期号：7

外文期刊名：MARINE DRUGS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000556429900001)】；

基金：This research was funded by the YNational Natural Science Foundation of China (31430002, 81573341, 21877038, 31720103901, 31320103911), Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), the Fundamental Research Funds for the Central Universities (22221818014), the Shandong Taishan Scholar Award to L.Z. the Australian Research Council (DP160101429, LE140100119, LE120100170).

语种：英文

外文关键词：Aspergillus fumigatus; genome mining; chemical diversity; antimicrobial activity; biosynthetic gene cluster; prenyltransferase

摘要：The marine-derived fungusAspergillusfumigatusMF071, isolated from sediment collected from the Bohai Sea, China, yielded two new compounds 19S,20-epoxy-18-oxotryprostatin A (1) and 20-hydroxy-18-oxotryprostatin A (2), in addition to 28 known compounds (3-30). The chemical structures were established on the basis of 1D, 2D NMR and HRESIMS spectroscopic data. This is the first report on NMR data of monomethylsulochrin-4-sulphate (4) and pseurotin H (10) as naturally occurring compounds. Compounds15,16,20,23, and30displayed weak antibacterial activity (minimum inhibitory concentration: 100 mu g/mL). Compounds18and19exhibited strong activity againstS. aureus(minimum inhibitory concentration: 6.25 and 3.13 mu g/mL, respectively) andE. coli(minimum inhibitory concentration: 6.25 and 3.13 mu g/mL, respectively). A genomic data analysis revealed the putative biosynthetic gene clustersftmfor fumitremorgins,psofor pseurotins,fgafor fumigaclavines, andhelfor helvolinic acid. These putative biosynthetic gene clusters fundamentally underpinned the enzymatic and mechanistic function study for the biosynthesis of these compounds. The current study reported two new compounds and biosynthetic gene clusters of fumitremorgins, pseurotins, fumigaclavines and helvolinic acid fromAspergillusfumigatusMF071.