文献类型：期刊文献

英文题名：Engineered synthesis of rosmarinic acid in Escherichia coli resulting production of a new intermediate, caffeoyl-phenyllactate

作者：Jiang, Jingjie[1,2];Bi, Huiping[2,3];Zhuang, Yibin[2,3];Liu, Shaowei[1];Liu, Tao[2,3];Ma, Yanhe[2]

机构：[1]E China Univ Sci & Technol, Sch Biol Engn, Dept Food Sci & Technol, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China;[3]Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China

年份：2016

卷号：38

期号：1

起止页码：81

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20153601241182);WOS:【SCI-EXPANDED(收录号:WOS:000367851600009)】；

基金：This work was supported by Grants from the 973 Program of China (No. 2012CB721100), the 863 Program of China (No. 2012AA02A704), the 100 Talents Program of the Chinese Academy of Sciences and the National Natural Science Foundation of China (31300040 and 21302214).

语种：英文

外文关键词：Biosynthesis; Caffeoyl-phenyllactate; 4-Coumarate CoA ligase; Escherichia coli; Rosmarinic acid; Rosmarinic acid synthase

摘要：To achieve high production of rosmarinic acid and derivatives in Escherichia coli which are important phenolic acids found in plants, and display diverse biological activities. The synthesis of rosmarinic acid was achieved by feeding caffeic acid and constructing an artificial pathway for 3,4-dihydroxyphenyllactic acid. Genes encoding the following enzymes: rosmarinic acid synthase from Coleus blumei, 4-coumarate: CoA ligase from Arabidopsis thaliana, 4-hydroxyphenyllactate 3-hydroxylase from E. coli and d-lactate dehydrogenase from Lactobacillus pentosus, were overexpressed in an l-tyrosine over-producing E. coli strain. The yield of rosmarinic acid reached similar to 130 mg l(-1) in the recombinant strain. In addition, a new intermediate, caffeoyl-phenyllactate (similar to 55 mg l(-1)), was also produced by the engineered E. coli strain. This work not only leads to high yield production of rosmarinic acid and analogues, but also sheds new light on the construction of the pathway of rosmarinic acid in E. coli.