文献类型：期刊文献

英文题名：The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription

作者：Fan, Jianhua[1];Cui, Yanbin[1];Zhou, Yang[1];Wan, Minxi[1];Wang, Weiliang[1];Xie, Jingli[1];Li, Yuanguang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：164

起止页码：214

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20142217778183);WOS:【SCI-EXPANDED(收录号:WOS:000338710500031)】；

基金：This work was supported by National Natural Science Foundation of China 31300295, National Basic Research Program of China 2011CB200904, China Postdoctoral Science Foundation 2013M541483, National Special Fund for State Key Laboratory of Bioreactor Engineering 2060204, the Fundamental Research Funds for the Central Universities, and Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Chlorella; Lipid; Gene expression; Heterotrophy; Photoautotrophy

摘要：Heterotrophy to photoautotrophy transition leads to the accumulation of lipids in Chlorella, which has potential to produce both healthy food and biofuels. Therefore, it is of key interest to study the metabolism shift and gene expression changes that influenced by the transition. Both total and neutral lipids contents were increased rapidly within 48 h after the switch to light environment, from 24.5% and 18.0% to 35.3% and 27.4%, respectively, along with the sharp decline of starch from 42.3% to 10.4% during 24 h photoinduction phase. By analyzing the correlation between lipid content and gene expression, results revealed several genes viz. me g3137, me g6562, pepc g6833, dgat g3280 and dgat g7566, which encode corresponding enzymes in the de novo lipid biosynthesis pathway, are highly related to lipid accumulation and might be exploited as target genes for genetic modification. These results represented the feasibility of lipid production through trophic converting cultivation. (C) 2014 Elsevier Ltd. All rights reserved.