文献类型：期刊文献

英文题名：Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

作者：Li, Hui[1];Shen, Ting-Ting[1];Wang, Xiao-Li[2];Lin, Kuang-Fei[1];Liu, Yong-Di[1];Lu, Shu-Guang[1];Gu, Ji-Dong[3];Wang, Ping[2];Lu, Qiang[1];Du, Xiao-Ming[4]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Univ Hong Kong, Swire Inst Marine Sci, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China;[3]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Chinese Res Inst Environm Sci, Dept Soil Pollut Control, Beijing 100012, Peoples R China

年份：2013

卷号：137

起止页码：286

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20131716231989);WOS:【SCI-EXPANDED(收录号:WOS:000319645600038)】；

基金：This research was financially supported by the National Natural Science Foundation of China (41003031, 41273109), the Shanghai Rising-Star Program (12QA1400800), the Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), the National Environmental Protection Public Welfare Science and Technology Research Program of China (200909089, 2010467032, 201109013), the Key Science Foundation of Shanghai Environmental Protection Bureau (Huhuanke2012-07), the Fundamental Research Funds for the Central Universities (WB1014004,WB1314002), the National Science Foundation for Postdoctoral Scientists of China (2012M510811). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：Co-contamination; Activated sludge; Enzyme activity; Microbial community diversity

摘要：This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150 mg/L) was mixed with 2-CP (in the range of 25-150 mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150 mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way. (C) 2013 Elsevier Ltd. All rights reserved.