文献类型：期刊文献

英文题名：An ultra-low noise amplifier array system for high throughput single entity analysis

作者：Zhong, Cheng-Bing[1];Ma, Hui[1];Wang, Jia-Jun[1];Zhang, Lin-Lin[1];Ying, Yi-Lun[1,2];Wang, Rong[3];Wan, Yong-Jing[3];Long, Yi-Tao[1]

机构：[1]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China;[2]Nanjing Univ, Chem & Biomed Innovat Ctr, Nanjing 210023, Peoples R China;[3]East China Univ Sci & Technol, Sch Informat Sci & Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：233

期号：0

起止页码：33

外文期刊名：FARADAY DISCUSSIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000730694100001)】；

基金：This research was supported by the National Natural Science Foundation of China (22027806, 22090050 and 61871183) and the Fundamental Research Funds for the Central Universities (2020102025S, 0205-14380258).

语种：英文

摘要：Electrochemical measurements at the single entity level provide ultra-sensitive tools for the precise diagnosis and understanding of basic biological and chemical processes. By decoding current signatures, single-entity electrochemistry provides abundant information on charges, sizes, shapes, catalytic performances and compositions. The accuracy of single-entity electrochemistry highly relies on advanced instrumentation to achieve the amperometric resolution at the sub-picoampere level and the temporal resolution at the sub-microsecond level. Currently, it is still a challenge for paralleling amplifiers to allow low-noise and high bandwidth single-entity electrochemical measurements. Herein, we developed a low-noise four-channel electrochemical instrumentation that integrates an Au electrode array with amplifiers in the circuit board. With this amplifier array, we achieved a high bandwidth (>100 kHz) electrochemical measurement. The further practical experiments proved the capability of this amplifier array system in acquiring transient signals from both single-molecule detection with an aerolysin nanopore and single Pt nanoparticle catalysis during the dynamic collision process. Paired with appropriate microfluidic array systems, our instrumentation will enable an extraordinarily high-throughput feature for single-entity sensing.